摘要
目的研究大豆异黄酮(SI)对去卵巢大鼠抗氧化及骨形态学的影响。方法70只雌性SD大鼠按血清总胆固醇水平随机分为7组:高脂、雌激素,低、中、高剂量SI干预、假手术及正常对照组。对前5组摘除双侧卵巢后恢复1周开始干预,实验周期为12周,灌胃给药,每周称重1次,分别在去卵巢及处死时(12周)抽取尾静脉血,实验结束后取内脏及骨骼标本,分别测定血清碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶活性(GSH-Px)及骨密度等指标,共63只大鼠完成实验。结果SI干预组股骨重量高于高脂与正常对照组,干预可提高AKP、GSH-Px酶活性。高剂量干预对维持大鼠股骨密度的作用与雌激素相似。SI干预可缓解因去卵巢造成的骨丢失。结论大豆异黄酮对去卵巢大鼠的抗氧化、骨代谢酶活性及骨转化存在一定影响,适量干预能逆转因去势造成的骨密度下降。考虑到植物雌激素与哺乳动物的雌激素受体(ER)结合能力低下,采用SI在临床开展干预的剂量与远期效果尚待进一步研究。
Objective To study the effects of soybean isoflavone （SI） on antioxidation and bone morphology in ovariectomized rats. Methods Seventy female Sprague-Dawley（SD） rats were randomly divided into 7 groups, according to the levels of total cholesterol in serum： high-lipoids group, estrogen group, low dose SI group, middle dose SI group, high dose SI group, sham group and normal control group. A week after bilateral ovaries were excised except in the sham and normal control groups estrogen, different doses of SI or deionized water were fed with intragastric administration for 12 weeks. Body weights were measured every week and venn caudalis serum, innards tissue, femoral bone were collected immediately after the rats were ovariectomized or killed. The activity of alkaline phosphatase（AKP）, superoxide dismutase（ SOD）, glutathione peroxidase（ GSH- Px） in serum and bone density were measured respectively, with 63 SD rats being used for the experiment. Results In the intervention groups femoral bone weights were significantly higher than those in both the high-lipoids and the control group. The activity of AKP.GSH-Px was increased after intervention, There was a same efficacy between high dose intervention and estrogen treatment on bone density. SI invention mitigated ovariectomy-induced bone loss. Conclusion The SI has some efficacy in ovariectomized rats antioxidation, activity of bone metabolism enzyme and can reverse bone loss. The decrease in bone density is interrupted under sufficient intervention. In view of phytoestrogen＇ s weak capability of binding with animals＇ estrogen receptor （ ER）, the dosage and the long-term effect of SI apphed in clinical intervention need further study.
出处
《解剖学报》
CAS
CSCD
北大核心
2007年第2期222-225,共4页
Acta Anatomica Sinica
基金
浙江省教育厅科研项目(20041049)
温州市科技局科研基金资助项目(Y2003A047)
关键词
大豆异黄酮
骨密度
骨代谢
组织化学
去卵巢大鼠
Soybean isoflavone
Bone density
Bone metabolism
Histochemistry
Ovariectomized rats
