摘要
目的:测定葛根芩连片中盐酸小檗碱和黄芩苷的含量。方法:采用高效液相色谱法,色谱柱为PhenomenexC18柱,流动相为甲醇-0.1mol·L-1磷酸二氢钾溶液(45:55),检测波长为264nm。结果:盐酸小檗碱在10.05mg/l～40.20mg/l质量浓度内、黄芩苷在25.20mg/l～100.80mg/l内呈良好的线性关系。两者的平均回收率分别为100.1%和100.5%。结论本方法可用于葛根芩连片的质量控制。
Objective： To determine the contents of berberine hydrochloride and baicalin in Gegen Qinlian tablets. Methods：A high-performance liquid chromatographic method was developed for the determination.A Phenomenex C18 column（150mm × 4.6mm,5 μm）was used, with the mobile phase of methyl：KH2PO4 （45：65）, at the detection wave length of 264nm. Result：The linear range of berberine hydrochloride was 10.05-40.20mg/l; the mean recovery was 100.1%;The linear range of baicalin was 25.2mg/l-100.8mg/l; the mean recovery was 100.5%. Conclusion： the method can be used in the qualitv control of Gegen Oinlian tablets.
出处
《中国伤残医学》
2007年第1期25-27,共3页
Chinese Journal of Trauma and Disability Medicine
