摘要
[目的]探讨内皮型一氧化氮合酶(eNOS)基因27bp数目可变的串联重复序列(VNTR)多态性与原发性高血压(EH)及其脑梗塞易感性的关系。[方法]①采用PCR-琼脂糖凝胶电泳法检测227例EH患者(其中123例无脑梗塞EH(-)、104例并发脑梗塞EH(+)和145例对照者27bpVNTR基因型;②硝酸还原酶法测定对照组空腹血清一氧化氮代谢物(NOx)水平。[结果]①EH组aa+ab基因型及a等位基因频率高于对照组(基因型0.220/0.083;等位基因0.121/0.041,P值均<0.01);EH(+)组与EH(-)组间基因频率分布类似。以65岁进行年龄分层,EH<65组aa+ab基因型及a等位基因频率高于对照组<65组(基因型0.256/0.073;等位基因0.140/0.036,P值均<0.01);EH(+)<65组aa+ab基因型及a等位基因频率高于EH(-)<65组(基因型0.347/0.194;等位基因0.194/0.104,P值均<0.05)。②多因素分析表明,27bpVNTR多态性是EH发生的独立危险因素,也是65岁以下EH患者发生脑梗塞的独立危险因素。③对照组aa+ab基因型空腹血清NOx低于bb基因型(P<0.05)。[结论]①eNOS基因27bpVNTR多态性可能与原发性高血压及其早发脑梗塞有关;②a等位基因可能通过减少内皮基础NO释放参与上述病变的发生。
[Objective] To explore the association of polymorphism of endothelial nitric oxide synthase （eNOS） gene 27 bp variable number of tandem repeats （VNTR） with essential hypertension （EH） and its susceptivity to cerebral infarction （CI）. [Method] ①Genotypes of 27 bp VNTR were determined by PCR-agarose gel electrophoresis in 227 EH patients [104 with CI-EH（ ＋ ） ;and 123 without-EH（ - ）] and 145 subjects in Group-control （CON）.②Fasting serum nitric oxide metabolites （NOx） were measured by nitrate reductase in CON, [Results] ① The frequencies of aa ＋ ab genotype and a allele of EH group was higher than those of CON （0. 220/0. 083;0. 121/0. 041,P 〈 0, 01 each）. The polymorphism distribution was similar between EH （ ＋ ） and EH （-）. In aged 〈65 ,the frequencies of aa ＋ ab genotype and a allele ofEH （EH〈65） was higher than those of CON （CON〈65）（0.256/0.073; 0.140/0.036,P 〈 0.01 each）. The frequencies of aa ＋ ab genotype and a allele of EH with CI（aged 〈 65 ）[EH（ ＋ ） 〈 65] was higher than those of EH without CI （aged 〈65） [EH（-）〈65] （0. 347/0, 194;0. 194/0. 104,P 〈 0.05 ）. ② Logistic analysis indicated that 27 bp VNTR polymorphism was independent risk factor of EH, 27 bp VNTR polymorphism was also independent risk factor of CI in patients of EH （aged ,65）.③ Fasting serum NOx of aa ＋ ab genotype was lower than that of bb genotype in CON （P 〈 0.05）. [Conclusion] ① The polymorphism of eNOS gene 27 bp VNTR is associated with EH and early CI. ② A allele may be involved in the etiology of those disease by reducing basal NO release of endothelium.
出处
《海峡预防医学杂志》
CAS
2007年第1期10-13,共4页
Strait Journal of Preventive Medicine
基金
福建省卫生厅青年科研基金资助项目(项目号:99-1-06)
关键词
原发性高血压
脑梗塞
内皮型一氧化氮合酶基因
数目可变的串联重复序列
Essential Hypertension （EH）
Cerebral Infarction （CI）
Polymorphism of Endothelial Nitric Oxide Synthase Gene
Variable Number of Tandem Repeat（VNTR）
