摘要
目的探讨内皮型一氧化氮合酶(eNOS)基因27bp数目可变的串联重复序列(VNTR)多态性与原发性高血压(EH)患者舒张压的关系及其可能机制。方法采用聚合酶链反应(PCR)及琼脂糖凝胶电泳检测278例EH病人的基因型,同时进行基因测序。用硝酸还原酶法测定空腹血清一氧化氮代谢物(NOx)浓度。结果aa+ab基因型EH患者的空腹血清NOx明显低于bb基因型(P<0.05);aa+ab基因型EH患者的舒张压显著高于bb基因型(P<0.05)。结论eNOS基因27bp串联重复序列(VNTR)的a等位基因可能通过减少NOx的释放而参与舒张压增高的形成。
Objective To investigate the relation between the polymorphism of endothelial nitric oxide synthase (eNOS) gene 27 bp variable number of tandem repeats (VNTR) and diastolic blood pressure in hypertensive patients and its possible mechanism. Methods Genotypes of 278 hypertensive patients were determined by polymerase chain reaction (PCR) and agarose gel electrophoresis and gene sequencing was performed at the same time; fasting serum nitric oxide metabolite (NOx) were measured by nitrate reductase. Results The levels of NOx in ab+aa genotype were lower than that in bb genotype(P<0.05). Diastolic blood pressures of ab+aa genotype were significantly higher than that of bb genotype(P<0.05). Conclusion It is suggested that the polymorphism of eNOS gene 27 bp VNTR may be involved in the etiology of the elevation of diastolic blood pressure by reducing NO release of endothelium.
出处
《首都医科大学学报》
CAS
2005年第3期309-311,共3页
Journal of Capital Medical University
基金
首都医学发展基金(2002-2007)资助项目
