摘要
目的建立炎症性肠病(IBD)患者和健康人肠道细菌 DNA 指纹图谱以分析其肠道菌丛的整体差异。方法应用肠杆菌基因间的重复共有序列(ERIC)-PCR 技术建立10例 IBD 患者及10例正常对照者的肠道细菌 DNA 指纹图谱,分析 IBD 患者及正常对照者的肠道菌丛的整体差异。结果 IBD 患者及正常对照者的肠道菌丛存在整体差异,正常对照组 ERIC-PCR 指纹图谱电泳 DNA条带多,主带位置无统一趋势;IBD 组 ERIC-PCR 指纹图谱电泳 DNA 条带较少,且所有样本主带分布非常一致,均出现在约1.1kb 处。结论正常人肠道细菌种类多,IBD 患者少。IBD 组1.1kb 处主带可能为某一种肠道细菌中特有的序列,或为不同序列或几个序列的混合物。
Objective DNA fingerprinting for inflammatory bowel disease （IBD） patients and healthy subjects was carried out to compare the difference of intestinal flora between the two groups. Methods DNA fingerprinting for IBD patients and healthy persons was set up with enterobacterial repetitive intergenic consensus （ERIC-PCR） technology and the difference of intestinal flora between the two groups compared. Results DNA fingerprinting of the IBD patients and healthy subjects was identified and a significant difference was noticed between them. There were lots of bands in the DNA fingerprinting of the healthy subjects but few in that of the IBD patients. Strikingly, same distribution of the principal band of DNA fingerprinting was noticed in IBD patients. Conclusions The variety of intestinal flora in healthy subjects is more apparent than that in IBD patients. An unique principal band might be the sequence of the presence of specific etiopathogenetic bacterium, or it might be the combined sequence of mixed bacterial flora.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2007年第2期96-98,共3页
Chinese Journal of Internal Medicine
