摘要
目的建立肠道疾病患者肠道细菌的DNA指纹图谱,并分析其肠道菌群结构特征的整体差异。方法选取消化科37例经结肠镜确诊的肠道疾病患者,包括溃疡性结肠炎(UC)患者20例、肠易激综合征(IBS)患者6例、急性胃肠炎患者11例,另取11名健康成人作为正常对照。提取粪便标本细菌总DNA,应用肠杆菌基因间重复共有序列基因扩增技术(ERIC-PCR)建立肠道菌群的DNA指纹图谱并分析其整体差异。结果UC患者样本DNA条带明显少于其他肠道疾病患者和正常对照,提示UC、急性胃肠炎、IBS和正常对照的肠道菌群存在整体差异。17例UC患者标本DNA主带出现在0.7kb处,11例急性胃肠炎患者样本在0.8kb和1.1kb处显示2条DNA主带,而正常对照和IBS患者样本DNA主带均无统一趋势。结论UC可能存在较单一的肠道优势菌群,其发病机制可能与特定的肠道细菌感染有关。
Objective To establish the DNA fingerprint of microflora of some intestinal diseases such as ulcerative colitis （UC）,acute gastroenteritis （AG） and irritable bowel syndrome （IBS）,and analyze the structural characteristics of such fingerprints. Methods Thirty seven patients with intestinal diseases,definitely diagnosed by coloscopy as UC （20 cases）,IBS （6 cases） and AG （11 cases）,and 11 healthy people as control were involved in the present study. The total DNA was extracted from the fecal samples,and enterobacterial repetitive intergenic consensus sequence-polymerase chain reaction （ERIC-PCR） was used to set up the DNA fingerprint of intestinal microflora. The differences existed among the fingerprint profiles of intestinal microflora were compared. Results The numbers of DNA bands were obviously less in UC patients than in IBS and AG patients and healthy subjects,implying that significant differences existed in the intestinal microflora among UC and AG patients and healthy subjects. The principal band of DNA fingerprint in 17 UC patients appeared at 0.7kb,and 2 main DNA bands existed at 0.8kb and 1.1kb in AG patients,while no principal band was found in the DNA fingerprint of the IBS patients and healthy subjects. Conclusions It is likely that a single principal microflora is presented in the intestinal tissue of UC patients,which might be responsible for the morbidity of UC.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2009年第11期1326-1327,1332,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助项目(30440072)
