摘要
目的对健康儿童与发育不佳(FTT)儿童肠道中微生物区系的ERIC-PCR指纹图谱异同进行研究。方法根据美国疾病预防控制中心(CDC)对儿童生长发育的评价指标对某幼儿园200例4～6岁儿童进行评价,筛选出16例健康儿童和13例FTT儿童,每周1次连续3周跟踪取样,提取粪便样品中细菌总DNA,获得其ERIC-PCR指纹图谱,再将其中一个样品的ERIC-PCR产物作为混合探针通过杂交对指纹图谱上DNA条带序列的异同进一步比较。结果同一个体的肠道菌群结构在取样期间稳定性较好;虽然健康儿童间的肠道菌群结构也有一定差异,但它们却有着共同的结构特征;而健康儿童与FTT儿童的肠道菌群结构差异较大。结论儿童发育状况与肠道菌群结构有一定的关系。
Objective To characterize the structural features of intestinal communities in fecal samples of healthy and FTT (failure to thrive) children.Methods 16 healthy children and 13 FTT ones were selected from 200 kids in a kindergarten. The fecal samples were collected from them once a week within three weeks. The genomic DNA of bacteria was extracted from each sample and ERIC-PCR amplification was then carried out. The PCR fingerprints were southern-blotted with a mixed probe from the labeled ERIC-PCR product of one sample.Results The banding-patterns of different samples from the same individuals were highly similar, indicating that the structure of microbial community in each individual was stable during the sampling period. ERIC-PCR fingerprints were different between the investigated individuals, even between different health children. However,a strong hybridization signal was commonly found in the fingerprints of samples from health children but no or weak signal was found in these from the FTT children.Conclusion The structure of intestinal microflora might be closely related to the development of children.
出处
《中国微生态学杂志》
CAS
CSCD
2005年第3期161-163,共3页
Chinese Journal of Microecology
