摘要
应用肠杆菌基因间重复一致序列聚合酶链反应技术(ERIC-PCR)对李斯特氏菌基因组DNA进行分析,结果显示,李斯特氏菌种间DNA指纹图谱带型差异较大;单核细胞增生性李斯特氏菌株间及相同血清型不同来源的菌株,其DNA指纹图谱带型也有明显差异。在单核细胞增生性李斯特氏菌各株的DNA指纹图谱中发现1600bp的种专一性扩增带。结果表明,ERIC-PCR技术可用于李斯特氏菌种、菌株的鉴定及进一步分型研究。
Enterobacteia repetitive intergenic consensus sequences-based PCR(ERIC-PCR) was used to generate DNA fingerprints for Listeria spp.We got the specific profiles with ERIC-PCR technique that enables to identify Listeria species and the L.monocytogenes strains of different sterotype,and the same sterotype of L.monocytogenes from different sources also could be identified.Moreover,the species-spcific 1600bp DNA fragment was obtained from the fingerprint of L.monocytogenes.The study indicates that ERIC-PCR technique can be used in the identification of Listeria species and strains and its further typing,which is simple and quickly.
出处
《遗传》
CAS
CSCD
北大核心
2003年第2期195-197,共3页
Hereditas（Beijing)
基金
辽宁省教委基金(9801211006)资助
关键词
肠杆菌
PCR
李斯特氏菌DNA指纹图谱
菌株鉴定
enterobacteia repetitive intergenic consensus sequences
PCR
Listeria spp.
DNA fingerprints
