摘要
建立了食品中单核细胞增生性李斯特菌快速、敏感、特异的聚合酶链反应PCR检测方法。选择的引物具有良好的单增李氏菌种的特异性。对人工污染在冷冻食品中单增李氏菌的检测低限是4－8CFU/10g，对其增菌液的检测低限是7.2－11×103/ml，每PCR反应体系的检测低限为86－132CFU。对400份自然污染样品的检测结果显示，PCR方法的检测结果同常规培养法的结果完全相符。
We established a rapid,sensitive and specific polymerase chain reaction(PCR)method for detection of Listeria monocytogenes(LM)in food.The selected primer could be amplified by PCR showing excellent features of LM. detection limit for LM in artificially contaminated food was 4－8 colony forming units(CFU)per 10g of food;enrichment limit was 7.2－11×103/ml;PCR reaction system imit was 86－132 CFU.400 naturally contaminated food samples were detected by both methods,and showed completely identical results.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2001年第5期52-55,共4页
Food Science
基金
国家出入境检验检疫局科学研究与技术开发重点基金!(K9514)
