摘要
目的:观察尿酸(UA)对体外培养的小鼠骨髓来源树突状细胞(BMDCs)成熟及免疫功能的影响。方法:体外分离培养小鼠骨髓来源的DC,使用GM-CSF、IL-4、LPS,并加入UA诱导培养DC。用流式细胞仪技术检测BMDCs细胞表面分子表达,用MTT法检测BMDCs与同基因小鼠T淋巴细胞的混合淋巴细胞反应;ELISA法测定DC培养上清IL-12的水平。结果:通过鉴定体外成功诱导培养出BMDCs。尿酸浓度为400mg/L或200mg/L时能增强DC细胞表面CD11c、CD83、CD86、IA/IE分子表达率;提高IL-12分泌水平(P<0.05);DC与T淋巴细胞比例分别为5∶1、10∶1、20∶1时,能增强DC刺激T细胞增殖能力(P<0.05);尿酸浓度为70mg/L时,细胞表面分子表达、IL-12分泌水平、刺激T细胞增殖作用与对照组比较均无明显差别(P>0.05)。结论:对体外培养诱导扩增的BMDCs,UA可促进分化与成熟,提高表面共刺激分子表达;增强其刺激T细胞增殖能力和IL-12分泌水平。UA的作用与浓度密切相关。
AIM： To investigate the effect of uric acid on the maturation and the biological function of murine bone - marrow derived dendritic cells （BMDCs） in vitro. METHODS： BMDCs were cultured with GM - CSF, IL - 4, LPS and uric acid. Cell phenotypes were analyzed by flow cytometry. MTT was used to detect the effect of uric acid on the function of BMDCs in stimulating the proliferation of T cells. IL - 12 released by BMDCs was also detected. RESULTS： BM- DCs were cultured and identified. Uric acid at concentrations of 200 mg/L and 400 mg/L increased the expression of the molecules （CDllc, CD83, CD86, IA/1E） on BMDCs surface and the IL- 12 level in the culture supernatants （P 〈 0. 05）, promoted the proliferation of T cells at the T： DC rate S： 1, 10： 1,20：1 （P 〈0.05）. However, uric acid at concentration of 70 mg/L had no effect on above molecule expression （ P〉0.05 ）, no effect on T cell proliferation with BMDCs （P〉0. 05） was observed. CONCLUSION： Uric acid promotes the differentiation, maturation, the expression of costimulatory molecules, the IL - 12 production in BMDCs and enhances the ability of BMDCs to stimulate the proliferation of T cell in a specical dose range.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第1期95-98,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30471533)
