摘要
目的观察尿酸对由HBV表面抗原表位肽负载的树突状细胞诱导免疫效应的影响。方法以负载肽S28-39的小鼠骨髓来源树突细胞,联合尿酸(uric acid,UA)尾静脉注射接种小鼠,每周1次,共2次。分为DC对照组,联合尿酸组,尿酸对照组,正常对照组。流式细胞仪法检测特异性CTL活性;ELISA法检测小鼠脾细胞培养上清液IFN-γ水平。结果体内S28-39特异性CTL,以联合尿酸接种组杀伤活性最强;联合尿酸接种组脾细胞IFN-γ分泌较对照组增高。结论尿酸能够增强乙肝表位肽负载的树突细胞诱导的S28-39特异性CTL杀伤活性;能促进免疫小鼠脾淋巴细胞分泌IFN-γ。尿酸具有增强负载S28-39树突细胞诱导的细胞免疫效应的活性。
Objective To evaluate the immune response in Balb/c mice immunized with uric acid and dendritic cells （DCs） pulsed with a HBsAg epitope peptide S28-29. Methods Balb/c mice were immunized with DC pulsed with the S28-29 peptide （S28-29-pulsed-DCs）, combined with uric acid. DCs and uric acid were injected through the tail vein of each mouse, and the immunization was repealed after 7 days. Cytotoxicity of specific CrLs in vivo was determined by the CFSE labeled spleen assay. Production of IFN-γ in the supematants of cultured splenoeytes was determined by using a commercial ELISA kit. Results The specific CTLs induced by S28-29 B-pulsed-DCs and uric acid had strong cytotoxicity to S28-29 Production of IFN-7 was increased in mice immunized with S28-29B-pulsed-DCs and uric acid. Conclusion Uric acid can promote the S28-29 specific cytotoxicity and the production of IFN-γ induced by S28-29 peptide pulsed-DCs;uric acid can enhance the cellular immune response induced by S28-29-pulsed-DCs.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2007年第1期26-29,共4页
Immunological Journal
基金
国家自然科学基金(30471533)
湖北省自然科学基金项目(2006ABA139)资助
