摘要
目的研究细脚拟青霉代谢物提取物(bioac-tive compounds from paecilomyces tenuipes,BCPT)的小鼠急性毒性及对慢性应激大鼠脑组织脂质过氧化的影响。方法采用慢性不可预见性应激(CUS)法建立大鼠抑郁模型;用紫外分光光度计测定大鼠脑组织中GSH-PX、CAT、SOD活性和MDA、NO含量。采用小鼠最大耐受量法,观察BCPT的急性毒性。结果BCPT能提高CUS大鼠脑组织中GSH-PX、CAT、SOD活性,降低CUS大鼠脑组织中MDA、NO含量;小鼠最大耐受量为9g·kg-1。结论BCPT毒性小,其抗抑郁作用机制可能与抗氧化有关。
AIM： To study the acute toxicity of bioactive compounds from paecilomyces tenuipes （BCPT） and the effect of BCPT on lipid peroxidation of brain in chronic stress model （CMS） of depression in rats. METHODS： The depression animal model was induced by chronic unforeseeable stress （CUS）. The maximally tolerated dose （MTD） was used to detect the acute toxicity of BCPT. UV spectrophotometer analysis technique was used to detect the activity of SOD, GSH-PX and CAT,and content of MDA and NO in rat＇s brain. RESULTS ： The MTD of BCPT was 9 g·kg^-1. BCPT could obviously enhance the activities of SOD, GSH-PX and CAT, and also significantly inhibit the increase of MDA and NO content in brain in CMS rats. CONCLUSION： BCPT has little toxicity and produces an antidespressant-like effect in antioxidation in CMS rats.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2006年第10期1137-1139,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
安徽省科技厅重点科研项目(№04023048)
安徽省教育厅重点科研项目(№2004KJ194ZD)
安徽省高校青年科研资助计划项目(№2006jq1096)
关键词
拟青霉
慢性应激
最大耐受量
脂质过氧化
paecilomyces
chronic stress
maximum tolerable dose
lipid peroxidation
