摘要
目的:优选出分离纯化柴胡总皂苷和姜黄素的最佳工艺条件。方法:采用紫外分光光度法以柴胡总皂苷为指标测定柴胡皂苷的含量,优选出最佳分离纯化条件。结果:DM130树脂对柴胡总皂苷和姜黄素的分离纯化最优,以3BV/h吸附速率吸附,4BV80%乙醇以4BV/h的流速进行洗脱效果最佳,柴胡总皂苷以柴胡皂苷a计精制度为516.62%,纯度为18.03%。姜黄素精制度为433.33%。结论:DM130大孔吸附树脂可用于柴胡郁金提取液中总皂苷和姜黄素的精制。
Objective ： To optimize the technological parameter of separation and purification of the total saponins Radix Bupleuri and curcumin. Methods ： With the extraction rate of the total saponin of Radix Bupleur as the assessment index, the optimized technological parameter of separation and purification was selected by UV Spectrophotometry. Results： DM130 was found to provide the best fit of separation and purification,take the best condition of 3BV/h current velocity, 80 % ethanol as the eluting reagent, and the eluting velocity was 4 BV/h. This method made the saponins total Radix Bupleuri have a refined degree of 516.62% and purification of 18.03% ,it also made the curcumin have a refined degree of 433.33%. Conclusion： DM130 resin could be used to refine the saponins total Radix Bupleuri and curcumin.
出处
《中华中医药学刊》
CAS
2009年第10期2123-2125,共3页
Chinese Archives of Traditional Chinese Medicine
基金
卫生部科学研究基金-浙江省医药卫生重大科技计划(WKJ2007-2-020)
浙江省自然科学基金项目(X206952)
浙江省中医药科技计划重点项目(2006Z001)
浙江省卫生高层次创新人才培养工程项目
关键词
柴胡
郁金
柴胡总皂苷
姜黄素
大孔树脂
Radix Bupleuri
Radix Curcumae
saponins total Radix Bupleuri
eurcumae
maeroporous adsorbents
