摘要
采用反相高效液相色谱法,一次进样,同时测定食品中常见的9种食品添加剂:苯甲酸、山梨酸、糖精钠、安赛密、柠檬黄、日落黄、胭脂红、苋菜红、咖啡因。实验采用Nova-PakC18柱(3.9mm×150mm,4μm,Waters)为分离柱,以20mmol/L乙酸铵(pH6.8)-甲醇为流动相(梯度洗脱:甲醇:5%5min;5%～30%,12.5%/min;30%继续3min;30%～5%,25%/min),采用紫外检测器在234nm下进行检测。整个分析过程在10min内完成。其平均加标回收率为77.0%～108.5%,相对标准偏差小于5%。该方法结果准确,适用于食品常规质量检测。
A novel method is proposed for the simultaneous separation and determination of night food additives, saccharin sodium, acesulfame potassium, benzoic acid, sorbic acid, carmine, amaranth, tartrazine, sunset yellow and caffeine by reversedphase high performance liquid chromatography. The separation was achieved within 10 rain by using a Nova-Pak reverse phase C18 column （150ram × 3.9mm, 4 p m, Waters Inc） with 20mmol/L NH4Ac （pH6.8）-methanol as mobile phase （gradient elution： methanol： 5% 5rain; 5%-30%, 12.5%/min; 30% continued 3rain; 30%-5%, 25%/min）, and lastly detected at 234nm through a UV detector. The average recoveries for real samples ranged from 77.0% to 108.5%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第11期457-461,共5页
Food Science
基金
集美大学中青年创新团队专项基金
关键词
食品
食品添加剂
高效液相色谱法
food
food additives
high performance fiquid chromatography
