摘要
目的观察化学去细胞异种神经移植后宿主许旺细胞向移植物内增殖的情况。方法移植化学去细胞兔神经修复大鼠1 cm坐骨神经缺损,4个月后处死动物,取移植物行苏木精-伊红染色、S-100免疫组织化学染色及透射电镜观察。结果再生神经纤维顺利长入移植物,围绕再生纤维有大量胞体S-100免疫组化反应阳性的细胞呈条索状排列,半薄切片显示移植物内再生轴突排列整齐,透射电镜显示有细胞包绕再生轴突并形成髓鞘。结论化学去细胞异种神经移植后宿主许旺细胞可随再生纤维长入移植物并形成髓鞘。
Objective To observed the immigration and proliferation of Schwann cells in acellular xeno-nerve graft in rats. Methods The sciatic nerves on the right side of the rats were exposed and 0. 8cm long segments of the nerves were removed from the mid-thigh level and replaced by 1. 0cm long rabbit nerves made aeellular through chemical extraction. After 4 months, the immigration and proliferation of Schwann cells in the graft were revealed by HE staining, S-100 immunohistochemical staining and transmission electromicroscope. Results In the rats repaired by acellular nerves, regenerated axons upgrow into the graft, and around regenerated axons there were abundant cells aligned, the cytoplasm of which were S-100 immunoreacrive. Electromicroscope observing showed that regenerated axons were surrounded by myelin formed by the migrated cells reoccupied the acellular segments. Conclusion The host Schwann cells can immigrate into rabbit nerve grafts made acellular through chemical extraction and form myelin enwrapping regenerated axons in rats.
出处
《中华显微外科杂志》
CSCD
北大核心
2006年第5期350-352,i0005,共4页
Chinese Journal of Microsurgery
基金
国家自然科学基金(30271338
30471746)
首都医学发展基金(2003-3080)。
关键词
去细胞异种神经
移植
神经再生
讦旺细胞
Acellular xenogeneous nerve
Graft
Nerve regeneration
Schwann cell
