摘要
目的：建立注射用大川芎（冻干）（川芎、天麻）中阿魏酸和天麻素的含量测定方法。方法：采用ODS柱，流动相分别为甲醇-水-冰醋酸（30：68：2）和水-冰醋酸（100：1），检测波长为320nm和270nm。结果：阿魏酸在0．0316～0．5056μg范围内呈良好的线性关系，平均加样同收率为98．43％、RSD为1．52％。天麻素在0．442～3．536μg范围内具有良好的线性关系，平均加样回收率为98．15％、RSD为1．68％。结论：本法简便、准确、重现性好，适用于该制剂的质量控制。
AIM： To set up a method for detennining ferulic acid and gastrodine in Dachuanxiong Hydrochloride for Injection（Rhizoma Chuanxiong, Rhizoma Gastrodiae）. METHODS： HPLC conditions consisted of ODS column, methanol-water-acetic acid （30 ： 68 ： 2） and water-acetic acid （100 ： 1） as mobile phases, detection wavelengths were at 320 nm and 270 nm. RESULTS： For ferulic acid, the linear range was within 0. 031 6- 0. 505 6 μg and the average recovery was 98.43% with RSD = 1.52%. For gastrodine, the linear range was within 0.442-3. 536μg and the average recovery was 98.15% with RSD = 1.68%. CONCLUSION： The method proves to be simple, precise and reproduciable and is suitable for the use of quantitative control of Dachuanxiong Hydrochloride for Injection.
出处
《中成药》
CAS
CSCD
北大核心
2006年第9期1281-1284,共4页
Chinese Traditional Patent Medicine
关键词
HPLC
注射用大川芎
阿魏酸
天麻素
HPLC
Dachuanxiong Hydrochloride for Injection.
ferulic acid
gastrodine
