摘要
目的建立以高效液相色谱法测定枳术颗粒中柚皮苷含量的方法。方法色谱柱为Shim-packCLC-ODS,流动相为乙腈-水(20∶80,磷酸调节pH值至3.0±0.1),检测波长为283nm,流速为1.0ml/min,柱温为室温。结果柚皮苷进样量在0.1208μg^3.0200μg范围内线性关系良好(r=0.9999);平均加样回收率为99.1%(RSD=2.0%)。结论本方法简便、准确、灵敏度高、分离度与重现性好,可用于本品的含量测定与质量控制。
OBJECTIVE： To establish a method for the content determination of Naringin in Zhishu granules by HPLC. METHODS;The Shim- pack CLC- ODS column was used .The mobile phase consisted of acetonitrile- water（20 ： 80, adjusted to pH value of 3.0± 0.1 with phosphoric acid） .The detection wavelength was set at 283nm, the flow rate at 1.0ml/min, and the column temperature at room temperature.RESULTS： The calibration curve of Naringin was in good linearity over the range of 0.1 208μg- 3.0 200μg （ r = 0.9 999） . The average recovery was 99.1% （RSD = 2.0% ） . CONCLUSION ： The present method is simple, accurate, sensitive as well as with good resolving power and reproducibility, which can be used for the content determination and quality control of Zhishu granules.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第14期1103-1104,共2页
China Pharmacy
