摘要
目的:探讨壳聚糖涂层并预置引导纤维神经导管修复周围神经缺损的效果。方法:18条杂交犬,平均分成3组,无菌条件下切断左侧胫神经,制成25mm的犬胫神经缺损模型。采用壳聚糖涂层并预置引导纤维的聚乳酸和聚羟基乙酸共聚物(PLGA)神经导管作为实验组A组,以单纯PLGA神经导管为B组,自体神经移植组为C组作对照,每组6条犬。术后12周后通过一般观察,肌电图检查,HE染色和S-100免疫组化观察,再生神经图像分析等评价修复的效果。结果:术后12周各组再生神经已通过神经导管长入远端,肌电图,HE染色和图像分析结果表明A组再生神经轴突数量及再生神经质量优于B组(P<0.05);C组优于A、B组。结论:壳聚糖涂层并预置引导纤维的神经导管能有效修复周围神经缺损。
Objective To study the result of using chitison nerve conduit on repairing tibial nerve defect of canine. Methods eighteen out-bred dogs were made the model of 25-mm gap on bilateral tibial nerve under sterility condition, canines were randomly divided into three groups（n=6）,Group A： polymer polyglycolic-lactic acid（PLGA） nerve conduit coated with chitison and filled with a guide-fiber as experimental group to repair 25 mm gap of canine tibial nerves. Group B： PLGA nerve conduit coated with chitison. Group C： autograft. GroupB, C acted as control group. The repair result was evaluated by normal observation,EMG testing, S-100 histological immunostaining and biography analysis at 12 weeks after operation. Results At 12 weeks, the regenerating nerve fibres were seen to have bridged the gap. The repair result of PGLA nerve conduit coated with a chitison and filled with guide-fiber was better than those of B groups,,but worse than C groups, Conclusion PGLA nerve conduit coated with chitison and filled with a guide-fiber offers a possible substitute for repair of peripheral nerve defects.
出处
《中国美容医学》
CAS
2006年第6期635-637,i0002,共4页
Chinese Journal of Aesthetic Medicine
基金
上海市科委招标课题资助项目(02DZ11007)
上海市自然科学基金资助项目(03ZR14075)
