摘要
目的:对三嵌段高分子骨组织工程支架材料--聚(丙交酯-乙交酯)犤天冬氨酸-聚乙二醇犦的生物相容性进行评价,探讨用于骨组织工程的可行性。方法:实验于2003-08/12在华中科技大学同济医学院附属协和医院骨科实验室完成。①过敏试验:20只豚鼠随机分为试验组、对照组,每只豚鼠脊柱两侧皮内注射等体积聚(丙交酯-乙交酯)犤天冬氨酸-聚乙二醇犦浸提液、生理盐水及体积分数为0.05的甲醛溶液,记录激发部位红斑水肿。②急性全身毒性试验:12只小白鼠随机分为试验组和对照组,该两组腹腔分别注射浸提液及生理盐水,4,24,48及72h观察动物的一般状态。③植入试验:聚(丙交酯-乙交酯)犤天冬氨酸-聚乙二醇犦植入6只新西兰大白兔肌肉内,1,4及8周切取材料周围0.5cm处肌肉组织,苏木精-伊红染色,光镜下观察。结果:①过敏试验结果:皮内注射浸提液及生理盐水动物皮肤无红斑水肿,注射体积分数为0.05的甲醛溶液出现中度以上红斑水肿。②急性全身毒性试验结果:阴性对照组未见毒性症状,试验组4h后仅1只有轻微的运动减少,24h后恢复正常。③植入试验结果:所有试验动物伤口均一期愈合。结论:聚(丙交酯-乙交酯)犤天冬氨酸-聚乙二醇犦具有良好生物相容性,是一种新的骨组织工程支架材料。
AIM：To evaluate the biocompatibility of poly （lactic acid/glycolic acid/ asparagic acid-co-polyethylene glycol） （PLGA-[ASP-PEG]） tfi-block copolymer in vivo so as to probe into the practicability as a scaffold for bone tissue engineering.
METHODS： The experiment was carried out at the Laboratory of Orthopaedic Department, Union Hospital , Tongji Medical College, Huazhong University of Science and Technology from August to December 2003. ① The sensitization test： The twenty guinea pigs were randomly divided into experimental group and control group. Leaching liquor from PLGA-[ASP-PEG], normal saline and 0.05 formaldehyde were injected intradermally into each side of the backbone of each guinea pig. The erythema and edema were recorded. ②The acute systemic toxicity test： The twelve mice were divided into experimental group and control group. Mice in the two groups were injected by leaching liquor and saline respectively abdominally, then the general state of the mice was recorded at the 4^th, 24^th, 48^th and 72^th hours. ③The implantation test： PLGA-[ASP-PEG] was implanted into the muscle of 6 New Zealand rabbits. Myoideum that was cut about 0.5 cm beside PLGA-[ASP-PEG] at 1, 4 and 8 weeks and were stained by hematoxylin and eosin and observed under light microscope.
RESULTS： ① The sensitization test： The erythema and edema failed to be found in the guinea pigs injected by leaching liquor and saline except for 0.05 formaldehyde. ②The acute systemic toxicity test： The symptom of toxicity was not found in negative control group. One mouse was found the slim hypokinesia at 4 hours; nevertheless, hypokinesia disappeared at 24 hours. ③The implantation test： the wound implanted by PLGA-[ASP-PEG] healed at first intention.
CONCLUSION： PLGA-[ASP-PEG] has good biocompatibility in vivo, so it can be used as a scaffold for bone tissue engineering.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第21期49-51,i0003,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助(30170270)~~
