摘要
目的 研究聚乳酸微丝在神经干内的降解特点以及在降解过程中对外周神经的影响 ,探讨其是否适合作为组织工程化外周神经的支架。 方法 在 2 0只SD大鼠两侧的坐骨神经内分别植入聚乳酸微丝 (实验侧 )和Vicryl微丝 (对照侧 ) ,于术后 1、4、8和 12周观察微丝的形态和神经组织结构的变化以及植入部位的组织和细胞反应。 结果 聚乳酸微丝植入神经干后在表面和内部同时发生崩裂 ,至 12周时已大部分降解 ;在降解过程中始终伴随着巨噬细胞活跃的吞噬活动 ;两种微丝植入神经干后均引起类似的轻度异物炎性反应 ;在聚乳酸微丝的降解过程中 ,神经束和神经纤维的组织结构没有明显变化。 结论 聚乳酸微丝在外周神经内的降解时间超过 12周 ;微丝在降解时引起的轻度非特异性炎性反应对神经组织没有不良影响。
Objective Investigate the degradation process of polyactic acid (PLA) filaments within peripheral nerves and its adverse effects on peripheral nerve. Method Bilateral sciatic nerves of 20 SD rats were exposed, and PLA filaments were implanted among the fascicles at one side, while Vicryl filaments were implanted into the contralateral nerve as control. The filaments and nerves were harvested at 1,4,8 and 12 weeks postoperatively, and inspected by macroscopy, light microscope and transmission electron microscope. Results The degradation occurred not only on the surface but also inside the PLA filaments after they were implanted into the sciatic nerve. PLA fragments still could be found at the end of this study. During the degradation of PLA filaments, macrophages invaded into the filaments and exhibited active phagocytosis. There were mild degree infiltration of lymphocytes and fibroblast around the filaments during their degradation. However, the structure of peripheral nerve didnt change significantly. Conclusion It takes more than 12 weeks that PLA filaments degrade in peripheral nerve completely. Mild nonspecific inflammation develops following the implantation of PLA filaments into peripheral nerve, but it has no adverse effects on nerve.
出处
《中华显微外科杂志》
CSCD
北大核心
2003年第1期33-35,共3页
Chinese Journal of Microsurgery
基金
国家自然科学基金 (39830 1 0 0
39870 80 5)
广东省自然科学基金 (980 0 57)
广东省医学科学技术研究基金 (B1 9990 37)
广州市科技攻关重大项目 (2 0 0 2Z1 E0 0 31 )
关键词
聚乳酸微丝
外周神经
降解性
药物载体
Polylactic acid
Degradation
Peripheral nerves
Tissue engineering
