摘要
目的:采用近红外光谱(NIR)技术对大青叶中的有效成分靛玉红的提取分离工艺进行研究。方法:采用氧化铝柱色谱法分离靛玉红,氯仿-醋酸乙酯作为洗脱剂,洗脱液用甲醇-丙酮重结晶。结果:此工艺所得靛玉红纯度为97.78%,且样品与对照品纯度相接近,NMR和IR图谱相同。结论:该法简便易行,安全、成本低、方便,实验结果良好。
AIM ： To study the suitable conditions of the extraction-separation process of the indirubin in Folium Isatidis with NIR. METHODS： The indirubin was chromatographed on an aluminium oxide column and chloroform-ethyl acetate as eluting agent, eluate was recrystalized by methanol-acetone. RESULTS： The indirubin extracted by this method was of high purity （97.78%）. The sample purity was similar to reference substance one （98.01%）. The spectra of NMR and IR are the same. CONCLUSION： This method is proved to be fast, safe, lower cost, simple and has good results.
出处
《中成药》
CAS
CSCD
北大核心
2006年第6期791-793,共3页
Chinese Traditional Patent Medicine
