摘要
在４份水稻材料的花药培养中，附加不同浓度硅素的试验结果表明，硅素能提高愈伤组织诱导率，绿苗分化率和每丛试管苗的绿苗数及绿苗移栽成活率。在所设６种处理中，以诱导培养基和分化培养基中均附加６０ｍｇ·Ｌ－１硅素的处理效果最好，０２４２８，０２４２８／ＩＲ３６和ＩＲ３６／０２４２８经该处理以后绿苗率分别比对照提高７３．９％、８６．６％和７９．６％。仅在分化培养基中附加硅素效果好于仅在诱导培养基中附加硅素处理。硅素对不同材料的作用方式不同，诱导培养基中附加硅素能提高０２４２８／ＩＲ３６和ＩＲ３６／０２４２８的愈伤组织诱导率和绿苗分化率，却不能提高０２４２８的愈伤组织诱导率，但在含硅培养基上诱导出的愈伤组织，其分化率较对照显著提高。硅素是影响水稻花培效率的重要因素，但不是决定因素。
When applied to 4 rice entries at various concentrations by addition to induction medium, differentiation medium or both of them, silicon was found baying effect of increasing callu induction frequency, plantlet differentiation frequency, green plantlet number per shoot cluster and transplanting survival rate in anther culture. The treatment of adding 60 mg. L- 1 silicon to both induction and differentiation media had the best effect among the six treatments, in this treatment the regeneration frequency of green plantlets for 02428,02428/IR36 and IR36/02428 significantly increased by 73. 9%, 86. 6 %and 79. 6 %,respectively, compared with the control. The treatment of adding 60 mg. L-1silicon to differentiation medium gave 13. 2% ～25. 5% more green plantlets than that to induction medium. Silicon in induction medium had different acting modes on different genotypes, so it might increase callus induction and plantlet differentiation frequencies of 02428/IR36 and IR36/02428 but was futile in increasing the callus induction frequency of 02428; however the calli induced from silicon-containing medium had a higher differentiation rate as compared to the control. Silicon is one of the major factors affecting rice anther culture, but isn' t a determinative factor.
出处
《上海农业学报》
CSCD
1996年第1期14-18,共5页
Acta Agriculturae Shanghai
关键词
硅素
水稻
花药培养
绿苗分化率
育苗
Silicon
Rice
Anther culture
Regeneration frequency of green plantlets
