摘要
在水稻花药培养中，用２０Ｇｙ剂量γ射线辐照诱导培养约３０天的早粳２６和舟优６号两个水稻品种花药和用每升含３０ｍｇ秋水仙碱的分化培养基培养愈伤组织，均可明显提高绿苗分化和再生。γ射线和秋水仙碱复合处理比各自单独使用具有更好的刺激效果。
The differentiation and regeneration of green plants from rice anther culture obviously increased when the anthers of rice varieties Zaojing 26 and Zhouyou 6 were irradiated with 20 Gy of γ ray at about 30days after culture on induction medium and calli were cultured on the differentiation medium containing 30 mg/L colchicine.The stimulation effect of γ irradiation combined with colchicine was much better than that of their single use.
出处
《浙江农业学报》
CSCD
1998年第4期184-187,共4页
Acta Agriculturae Zhejiangensis
关键词
Γ射线
秋水仙碱
水稻
花药培养
绿苗再生
rice anther culture
γ irradiation
colchicine treatment
stimulation effect
green plant regeneration
