摘要
目的探讨原发性胆囊癌组织中细胞凋亡及相关基因蛋白Bcl-2及p21WAF1的表达。方法用免疫组化ABC法检测46例原发性胆囊癌不同组织学分级中p21WAF1和Bcl-2的表达,TUNAL法检测细胞凋亡的发生。结果在原发性胆囊癌中随着组织学分级的增加,细胞凋亡率减少,并且p21WAF1表达减弱,而Bcl-2表达增强,差异均有统计学意义(P<0.05);相关分析显示细胞凋亡率与p21WAF1表达呈正相关(r=0.52,P<0.05),与Bcl-2表达呈负相关(r=-0.58,P<0.05)。结论细胞凋亡对原发性胆囊癌的发生发展有着重要影响,p21WAF1及Bcl-2基因参与胆囊癌的发生和发展。
Objective To investigate the effects of apoptosis and related gene p21^WAF1 and Bcl - 2 in the tumorigenesis of primary gallbladder neoplasms. Methods p21^WAF1 and Bcl - 2 were detected by immunohistochemistry and apoptotic ceils were stained in situ by terminal deoxynudeotidyl transferase mediated- dUTP nick end labeling（TUNAL） in 46 cases of primary gallbladder neoplasms with different histological grades. Results Apoptotic index and p21^WAF1 were decreased, but the expression of Bcl-2 was increased with the increasing of the histological grades in primary gallbladder neoplasms （P〈0.05）. p21^WAF1 expression was positively and Bcl-2 expression was negatively associated with the rateof apoptosis（r =0.52,P〈0.05; r= -0.58,P〈0.05 respectively）. Conclusion It indicates that apoptosis has an important role in the tumorigenesis of primary gallbladder neoplasms, p21^WAF1 and Bcl-2 take part in the occurrence and progression of primary gallbladder neoplasms.
出处
《中国医师进修杂志》
2006年第4期7-8,24,共3页
Chinese Journal of Postgraduates of Medicine
