摘要
目的优化基因工程菌Escherichia coli BL 21生产人源型胶原蛋白的高密度发酵工艺。方法建立一套以溶解氧浓度和比生长速率为中心的控制策略,采用溶氧反馈或pH反馈调节补料速度。结果 OD600可达150,胶原蛋白的表达量为9g／L。结论 20％-30％饱和度的溶解氧和0．1- 0．2 h-1的比生长速率有利于细胞生长和产物表达,其与降低乙酸等有害代谢副产物的积累密切相关。
Aim To study and optimize the process of the high cell-density fermentation of recombinant E. coli BL 21 and the expression of the human-like collagen. Methods A strategy in which the dissolved oxygen （DO） and specific growth rate （μ） were as the key parameters was applied. The glucose feeding control was DO and pH- stats, which is aimed to suppress the metabolic activity of acetic acid synthesis. Results Finally, the A600 （ OD600 ） value reached 150 and the collagen with a yield of 9 g/L was obtained. Conclusion It was beneficial to the cell growth and aim protein expression when the dissolved oxygen was controlled at 20% -30% and the specific growth rate at 0.1 -0.2 h^-1. And it could reduce the accumulation of acetic acid.
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第2期227-230,共4页
Journal of Northwest University（Natural Science Edition）
基金
国家科技攻关重大专项基金资助项目(2003DA901A32)国家自然科学基金资助项目(20476085)
关键词
重组大肠杆菌
高密度发酵
乙酸
类人胶原蛋白
recombinant Escherichia coli
high cell-density fermentation
acetic acid
human-like collagen
