摘要
目的实现TRAIL工程菌的高密度发酵,并测定TRAIL在体内的抗瘤活性。方法利用自控发酵罐,采用恒定溶氧、连续补料的方法对TRAIL工程菌进行了高密度发酵,从发酵菌体中纯化TRAIL蛋白,构建动物肿瘤模型,用所纯化的蛋白进行体内抗瘤试验。结果高密度发酵取得成功,发酵菌中TRAIL的表达量与摇瓶中的相当,摇瓶水平纯化TRAIL蛋白的工艺也适用发酵水平,体内试验显示TRAIL对小鼠移植瘤的抑制率达到67.2%。结论TRAIL工程菌成功实现高密度发酵,TRAIL蛋白在体内具有明显的抗瘤活性。
Objective To realize high cell-density fermentation of TRAIL engineered E.Coli, and to evaluate the anti-tumor activity of TRAIL in vivo. Methods High cell-density fermentation of TRAIL engineered E.Coli was carried out with constant soluble oxygen and continuous cultivation in self-control fermentor, the bioactivity of purified recombinant TRAIL protein was evaluated in animal model of tumor which constructed by us. Results High cell-density fermentation of TRAIL engineered E.Coli was succeeded, and the expression of TRAIL in fermented bacterial was the same as the flask culture. The inhibition rate of tumor by TRAIL was much as 67.2%. Conclusion High cell-density fermentation of TRAIL engineered E.Coli was realized and TRAIL can significantly inhibit the growth of transplanted tumor.
出处
《山西医科大学学报》
CAS
2005年第2期139-142,共4页
Journal of Shanxi Medical University
关键词
发酵
体内活性
TRAIL
fermentation
bioactivity,in vivo
TRAIL
