摘要
目的：观察大豆异黄酮对心肌梗死大鼠心室重构的影响。方法：雄性SD大鼠,结扎冠状动脉左前降支,造成心肌梗死。实验分为6组：假手术组,心肌梗死模型组,卡托普利组,大豆异黄酮低、中、高剂量组。在结扎冠状动脉后3h开始,分别灌胃给予0.5%羧甲基纤维素钠(CMC-Na),0.5%CMC-Na,卡托普利50mg·kg^(-1)和大豆异黄酮30,90,270 mg·kg^(-1),qd,共35 d。给药容积均为10 mL·kg^(-1)。最后一次给药后24 h,测定心率(HR)、左室舒张末期压(LVEDP)、左室收缩压峰值(Peak)、左室压最大上升速率/下降速率(±dp/dt_(max))和心肌最大缩短速率(V_(max)),测定全心室质量和体重比(TVW/BW),用天狼猩红染色,在图像分析系统下测量梗死范围(IS)、左心室内径(LVD)、室间隔厚度(ST),心肌间质胶原容积系数(ICVF)和血管周围胶原容积系数(PCVF)。结果：卡托普利、大豆异黄酮90 mg·kg^(-1)和270 mg·kg^(-1)组大鼠的梗死范围分别是(31±s4)%,(32±3)%和(31±5)%,均显著小于心肌梗死模型组大鼠[(38.9±2.9)%,P＜0.01]。心肌梗死组大鼠的TVW/BW,LVD,ICVF和PCVF均比假手术组明显增加(P＜0.01),增加的TVW/BW,LVD,ICVF和PCVF用卡托普利和大豆异黄酮90 mg·kg^(-1),270 mg·kg^(-1)治疗后明显减少[P＜0.01,P＜0.05(SI 90 mg·kg^(-1)组的LVD)]。心肌梗死大鼠的ST显著减小(P＜0.01),而给予卡托普利和大豆异黄酮可增加ST(P＜0.01)。结扎LAD后,大鼠的Peak,±dp/dt_(max)和V_(max)明显降低(P＜0.01),而LVEDP明显升高(P＜0.01),降低的Peak,±dp/dt_(max)和V_(max)在给予卡托普利、大豆异黄酮90 mg·kg^(-1)和270 mg·kg^(-1)后明显升高(P＜0.01)；而升高的LVEDP仅在给予卡托普利治疗后降低。结论：大豆异黄酮能够改善心肌梗死大鼠的心功能和心室重构。
AIM： To investigate the effect of soybean isoflavones （SI） on ventricular remodeling induced by myocardial infarction （MI）. METHODS： MI was induced bv permanent ligation of the left anterior descending coronary artery CLAD） in male Sprague-Dawley （SD）rats. Rats were randomly divided into six groups： shamoperated group （sham group）, MI model group, captopril 50 mg·kg^-1 group and SI 30, 90, 270 mg·kg^-1 groups. Three hours after the operation, the drugs or solvent were administrated ig qd for 35 d. Twenty-four hours after the last administration, heart rate （HR）, left ventricular end-diastolic pressure （LVEDP）, peak systolic left ventricular pressure （Peak）, maximal rate of left ventricular pressure rising （＋dp/dtmax）, maximal rate of left ventricular pressure declining （-dp/dtomax） and myocardial maximal shortening velocity （Vmax） were measured. After above hemodynamic parameters were measured, hearts were extracted. The ratio of total ventricle weight to body weight（TVW/BW） was calculated. Myocardial collagen was shown with the collagen-specific picrosirius red stain, myocardial interstitial collagen volume fraction（ICVF）, perivascular collagen volume fraction（PCVF）, infarct size, septal thickness （ST） and left ventricular diameter （LVD） were measured by image analysis system. RESULTS： The infarct size of rats in captopril, SI 90mg·kg^-1 and SI 270mg·kg^-1 groups were （31±s4） %, （32±3） %, and （31±5） %, respectively, all of which were significantly smaller than that in MI group （（ 38.9±2.9） %, P 〈 0.01 ）. In MI group, TVW/BW, LVD, ICVF and PCVF were remarkably increased compared with those in sham group （P 〈 0.01 ）. The increased TVW/BW, LVD, ICVF and PCVF were significantly reduced by treatment with captopril, SI 90mg·kg^-1 or SI 270 mg·kg^-1 （P 〈 0.01, P 〈 0.05 （LVD in SI 90 mg·kg^-1 group））. Whereas, ST of rats in MI group was significantly decreased than that in sham group （P 〈 0.01 �
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2006年第4期241-247,共7页
Chinese Journal of New Drugs and Clinical Remedies
关键词
异黄酮类
黄豆
心肌梗死
心室复建
血流动力学
大鼠
isoflavones
soybeans
myocardial infarction
ventricular remodeling
hemodynamics
rats
