摘要
目的研究内皮素(Endothelin-1,ET-1)与血管内皮生长因子(Vascular endothelial growth factor,VEGF)在肺癌的阳性表达、影响肺癌生长转移的作用机制及其相互作用关系。方法应用免疫组化SABC染色法检测40例原发性支气管肺癌标本中ET-1、VEGF的阳性表达、微血管密度(Microvessel density,MVD)计数,分别评价ET-1和VEGF与非小细胞肺癌临床病理参数之间的关系、ET-1和VEGF的关系;采用图象分析技术测定ET-1含量。结果ET-1和VEGF在肺癌组织中呈高阳性表达,二者阳性表达者的MVD值均明显高于VEGF阴性者,差异有统计学意义。ET-1在非小细胞肺癌组织中呈现阳性高表达,其含量及阳性表达与肿瘤分化程度、大小和淋巴结转移密切相关。分化程度越低,肿瘤直径越大、已有淋巴结转移,则ET-1含量和表达越高。在非小细胞肺癌VEGF阳性率与肿瘤大小,淋巴结转移与否密切相关。ET-1表达阳性区域,VEGF表达也呈阳性,在两者皆表达阳性的癌组织中MVD值明显高于单一阳性表达的肺癌组织,差异具有统计学意义。结论(1)ET-1在促进非小细胞肺癌血管生成的过程中发挥重要作用,可作为评估非小细胞肺癌恶性程度、淋巴结转移潜能和预后的重要监测指标之一;(2)ET-1的表达与VEGF有关,可能对VEGF具有上调作用,二者相互协同,共同调节和诱导肺癌微血管的生成。
Objective To study positive expression of endothelin - 1 （ ET- 1）, relationship between the ET - 1 and vascular endothelial growth factor （VEGF）, and discuss the role of ET- 1 in neovascularzation of lung cancer. Methods ET- 1 and VEGF positive expressions were detected by immuno - histochemistry and image analysis. Results ET - 1 positive expressions were mainly located in the cytoplasm of lung cancer. The positive rate of ET- 1 in non - small cell hing cancer was significantly higher than that in small - cell lung cancer. The larger the tumor, the lower the differentiating grade, and the more extensive the metastasis of non- small cell lung cancer was, the higherasas ET - 1 positive expression. Microvessel demity was significantly higher in the tissue specimens of both ET- 1 and VEGF positive expressions. Condusion ET- 1 plays an important rolle in the process of growth and metastasis of non - small cell lung cancer. ET- 1 may lead to VEGF overexpression in lung carcinoma, which contributes to neovascalarization of non - small cell lung cancer as an angingenic regulator.
出处
《武警医学》
CAS
2006年第3期188-192,共5页
Medical Journal of the Chinese People's Armed Police Force
关键词
非小细胞肺癌
内皮素
血管内皮生长因子
微血管密度
Non- small hing cancer Endothelin - 1 Vasanlar endothelial growth factor Microvessel demity
