摘要
目的:探讨VEGF、P53、PCNA、MVD在非小细胞肺癌(NSCLC)的表达及其生物学行为的关系。方法:采用免疫组织化学方法,对病理确诊的62例非小细胞肺癌组织进行VEGF,PCNA,P53和MVD表达检测。结果:62例非小细胞肺癌VEGF阳性表达率58.1%(36/62),其表达与NSCLC的组织分化程度相关(P<0.05)。P53阳性表达率46.8%(29/62),其表达与NSCLC的组织分化程度相关(P<0.05),PCNA阳性表达率61.0%(36/59),其表达与NSCLC的组织分化程度有关(P<0.05)。MVD值与肿瘤分化程度有关(P<0.05),VEGF,P53,PCNA和MVD均与肿瘤组织类型、TNM分期、有无淋巴结转移无关。结论:NSCLC中VEGF,PCNA,P53和MVD表达与NSCLC的发生、发展、转移及预后起着重要的作用,可作为评价非小细胞肺癌预后的标志物。
Objective：To investigate the expression of vascular endothelial growth factor（VEGF）,P53,PCNA and microvascular density（MVD） in non-small cell lung cancer and normal lung tissue and their correlation on biological behaviors.Methods： Expression of VEGF,P53,PCNA and MVD were detected by immunohisto chemistry method in 62 cases of NSCLC,the relationship between such expression and clinicopathological features of NSCLC was also studied.Results： The positive immunostaining rate for VEGF was 58.1%（36/62）,whose expression was associated with tumor differentiation（P0.05）.The positive immunostaining rate for P53 was 46.8%（29/62）,the expression of P53 was associated with tumor differentiation（P0.05）.The positive immunostaining rate for PCNA was 61.0%（36/59）,the expression of PCNA was associated with tumor differentiation（P0.05）.The value of MVD was associated with tumor differentiation（P0.05）.There were no relation to lymph node metastasis,TNM stage and pathological types of cancers.Conclusion： VEGF,PCNA,P53 and MVD play important roles in the development,progression,metastasis and prognosis of NSCLC.They may be new markers for prognosis of NSCLC.
出处
《中国卫生检验杂志》
CAS
2011年第7期1730-1732,共3页
Chinese Journal of Health Laboratory Technology
基金
黑龙江省教育厅科研项目(1151150)
关键词
非小细胞肺癌
血管内皮生长因子
微血管密度
增殖细胞核抗原
NSCLC
Vascular endothelial growth factor（VEGF）
Microvessel density（MVD）
Proliferating cell nuclear antigen（PCNA）
