摘要
肿瘤发生和发育畸形与染色体的不平衡有关,包括染色体的扩增和缺失。常规的比较基因组杂交技术(conv-entional comparative genomic hybridization)由于能在一次试验中掌握整个基因组DNA拷贝数的变化并可以将此改变准确的定位于染色体,因而在探讨肿瘤的发生和生长发育畸形机理的研究方面得到了广泛的应用。在此基础上发展起来的微阵列比较基因组杂交技术(microarray CGH/array-based CGH),为精确、定量地研究人类基因组微缺失和微扩增及其定位提供了有力工具。本文综述了CGH和CGHa技术的原理及应用。
The majority of human tumors as well as many developmental abnormalities harbor chromosomal imbalances, many of which restdt from the gain and/or loss of genomic material. Conventional comparative genomic hybridization （CGH） has been used extensively to map DNA copy number changes to chromosomal positions. The pecent introduction of microarray comparative genomic hybridization （CGHa） provides a powerful tool to precisely detect and quantify genomic subtle deletions or duplications. This paper reviews the principle and application of CGH and CGHa.
出处
《中国优生与遗传杂志》
2005年第12期1-3,共3页
Chinese Journal of Birth Health & Heredity
关键词
比较基因组杂交
染色体
肿瘤
畸形
Comparative genonfic hybridization
Chromosome
Tumor
Abnormality
