摘要
目的:检测食管癌高发区河南省林州市食管鳞癌及相应癌旁正常上皮组织的脆性组氨酸三联体(fragilehistidinetriad,FHIT)蛋白和mRNA表达情况,从而探讨FHIT与该地区食管癌发生发展的关系.方法:采用卵白素-生物素-辣根过氧化物酶复合物(ABC)免疫组织化学染色法检测了42对食管癌高发区河南省林州市手术标本的癌组织和癌旁组织中FHIT蛋白表达水平.采用反转录PCR方法检测了16对食管癌和癌旁正常组织中FHIT基因mRNA表达状况.结果:FHIT蛋白在食管癌组织中缺失表达(24/42,57.1%)或弱表达(18/42,42.9%),与癌旁正常上皮组织中FHIT蛋白情况(40/42,95.2%)差异显著(P<0.05).低分化癌组织中FHIT蛋白阴性表达率为(13/15,87%),分化癌组织阴性表达率为(11/27,41%),二者有显著性差异(P<0.05).癌组织FHITmRNA表达阳性率(4/16,25%)显著低于癌旁组织(12/16,75%)(P<0.05).结论:FHIT基因可能在该地区食管癌的发生发展过程中起重要作用,是重要的抑癌蛋白,其表达减弱或缺失增加了食管癌的易感性.
AIM: To investigate the mRNA and protein expression of fragile histidine triad (FHIT) in human esophageal carcinoma and adjacent normal tissues in high-risk area in Linzhou city, Henan province. METHODS: The expression of FHIT mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in 16 cases of esophageal squmous cancer and matched adjacent cancer tissues, and the expression of FHIT protein was determined by immunohistochemistry in 42 cases. RESULTS: The expression of FHIT protein was found absent in 57.1 % (24/42) of tumor samples and weak in 42.9% (18/42), which was significantly higher than that in cancer-adjacent tissues (95.2%, 40/42)(P<0.05). FHIT mRNA expression in cancer tissues (4/16,25%) was markedly lower than that in cancer-adjacent tissues (12/16,75%)(P<0.05). CONCLUSION: FHIT protein may be the important tumor suppressor protein carcinogenesis and progression of esophageal carcinoma. Its lower or absent expression may increase the incidence of esophageal carcinoma in the high-risk area.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第12期1417-1420,共4页
World Chinese Journal of Digestology
关键词
河南
食管癌高发区
癌旁正常组织
FHIT蛋白
MRNA
表达
Esophageal carcinoma
Fragile histidine triad
Reverse transcriptase-polymerase chain reaction
Immu-nohistochemistry
