摘要
目的:探讨脆性组氨酸三联体(FHIT)基因和Ki-67抗原在原发性胆囊癌、胆囊腺瘤和慢性胆囊炎组织中的表达情况.方法:应用免疫组织化学S-P法,检测51例原发性胆囊癌、15例胆囊腺瘤和12例慢性胆囊炎组织中FHIT和Ki-67的表达情况,并分析其与胆囊癌临床病理因素的关系.结果:胆囊癌组中FHIT阳性率明显低于胆囊腺瘤和慢性胆囊炎组(47.1%vs66.7%,91.7%,P<0.01),而Ki-67阳性率明显高于胆囊腺瘤和慢性胆囊炎组(82.4%vs20.0%,0,P<0.01).FHIT表达缺失不仅与胆囊癌的高分级(18.8%vs56.5%,66.7%,P=0.02)、淋巴结或远处转移(33.3%vs66.7%,P=0.019)明显相关,而且与术后生存时间减少关系密切(31.2%vs77.8%,P=0.041).Ki-67表达与胆囊癌的高分级(58.3%vs87.0%,93.8%,P=0.039)和淋巴结或远处转移(33.3%vs66.7%,P=0.037)明显相关.FHIT与Ki-67表达呈负相关(r=-0.285,P=0.043).结论:FHIT基因是胆囊癌的一个候选抑癌基因.FHIT和Ki-67在胆囊癌的发生和演化中均起着重要作用,联合检测FHIT和Ki-67的表达情况有助于临床评估胆囊癌的生物学行为和判断预后.
AIM： To investigate the expression of fragile histidine triad （FHIT） gene and Ki-67 antigen in primary gallbladder carcinoma, gallbladder adenoma and chronic cholecystitis.
METHODS： Streptavidin-peroxidase immunohistochemical method was used to determine the expression of FHIT and Ki-67 in 51 cases of primary gallbladder carcinoma, 15 cases of gallbladder adenoma and 12 cases of chronic cholecystitis. Their relationships with clinical and pathological features of gallbladder carcinoma were analyzed.
RESULTS： The positive rate of FHIT expression was significantly lower in gallbladder carcinoma than that in gallbladder adenoma and chronic cholecystitis （47.1% vs 66.7%, 91.7%, P 〈 0.01）, while the positive rate of Ki-67 expression was markedly higher than that in gallbladder adenoma and chronic cholecystitis （82.4% vs 20.0%, 0, P 〈 0.01）. Loss of FHIT expression was significantly correlated with the higher histological grade （18.8% vs 56.5%, 66.7%, P = 0.02） and lymph node or distant metastasis （33.3% vs 66.7%, P = 0.019）, as well as survival time after operation （31.2% vs 77.8%, P = 0.041）. The expression of Ki-67 was increased with the higher histological grades （58.3% vs 87.0%, 93.8%, P = 0.039） and lymph node or distant metastasis （33.3% vs 66.7%, P = 0.037）. There was a negative correlation between FHIT and Ki-67 （r = -0.285, P = 0.043）.
CONCLUSION： FHIT may be a candidate tumor suppressor gene of gallbladder carcinoma. FHIT and Ki-67 play significant roles in the pathogenesis and evolution of gallbladder carcinoma. The combined detection of FHIT and Ki-67 protein may be helpful for clinical assessment of tumor biological behavior and prognosis in patients with gallbladder carcinoma.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第35期3382-3386,共5页
World Chinese Journal of Digestology
