摘要
利用作者构建的植酸酶基因工程菌于产酶培养基中降解植酸及其盐类,释放出有效磷,每隔12 h对有效磷含量进行测定,并计算相应酶活力,60 h和84 h时有效磷含量的增加出现两个峰值,此时酶活力分别达到1.453×105U/mL和1.501×105U/mL.并研究了该植酸酶基因表达菌株的产物对玉米生长的影响,采用9种不同处理的玉米培养液进行玉米培养试验,培养10 d后测定玉米幼苗的形态特征参数.结果表明,不同处理的玉米培养液显著影响玉米幼苗的根长、根部重量、根部以上部分植株高度、重量.施加28 mL菌体裂解液的玉米培养液,促进玉米幼苗生长的效果显著高于其它处理.可见,该菌株能很好地表达活性植酸酶,并在其生长代谢作用下降解植酸及其盐类,所释放的有效磷对植物生长具有良好的促进作用.
In this work, phytase engineering strain constructed previously was used to hydrolyze phytic acid and phytate to myo-inositol and inorganic phosphate in the medium. After every 12 h, effective phosphorus was tested and enzyme activity was calculated. Two peaks that locate at 60 h and 84 h shows 1. 453 ×10^5U/mL and 1. 501×10^5U/mL of enzyme activity. The corn was selected to test the function of this strain, and was cultured with 9 different kinds of culture liquids for 10 days. Then the results of shape parameter showed that heights and weights over roots, lengths and weights of roots of seedlings were notably affected by different culture liquids. The parameters of culture liquids including 28 mL lytic liquids were bigger than others. The strain can express phytase activity and hydrolyzes phytate to myo-inositol and inorganic phosphate which promotes the growth of plants.
出处
《天津师范大学学报（自然科学版）》
CAS
2005年第4期24-27,共4页
Journal of Tianjin Normal University：Natural Science Edition
基金
天津市科技攻关项目(0303122111-4)
天津市自然基金资助项目(013609811)
关键词
植酸
植酸酶
磷
玉米苗
phytate
phytase
phosphorus
corn seedling
