摘要
目的介绍雪旺细胞体外培养、纯化的新方法。方法用0.25%Ⅳ型胶原酶和0.03%胰蛋白酶消化出生3 d的SD鼠臂层神经和坐骨神经组织块40 min,再将组织块贴壁培养。然后采用组织块反复种植纯化法、低浓度胰酶快速消化和差速贴壁法对雪旺细胞进行纯化。结果雪旺细胞从组织块中“爬出”速度较直接组织贴壁培养方法快,且纯度可达98%以上,细胞所受到的外界因素影响也较少。结论先用酶消化后组织块贴壁培养雪旺细胞的方法和组织块反复种植纯化、低浓度胰酶快速消化和差速贴壁法综合运用是一种简便有效的细胞培养、纯化方法,值得进一步推广。
Objective To introduce a new way for culturing and purifying schwann cell in vitro. Methods The sciatic and brachial plexus nerve of three-day-old SD mice was treated with 0.25 % collagenase type Ⅳ and trypsin for fourty minutes to remove the desmocyte on the schwann cell, then explanted in the culture disk. The schwann cell were purified by repeated explantation,rapid trypsinization,and differential adhesion. Result Schwann cell was purer and grew faster from the treated nerve than the nerve without treating,and little affected by non-experimented factors. Conclusion It is a simple, availability, worth spreading way to obtain and purify the schwann cell by the upper.
出处
《中国基层医药》
CAS
2005年第10期1299-1300,共2页
Chinese Journal of Primary Medicine and Pharmacy
基金
广东省医学科研基金项目(B2001065)
