摘要
目的:许旺细胞能分泌生长因子,在周围神经再生中起重要作用,获得高纯度许旺细胞是对其研究的重要前提,为此探讨获得高纯度的SD乳鼠的许旺细胞的有效方法。方法:组织块反复种植纯化法、低含量胰酶快速消化和差速贴壁法。结果:经组织块反复种植纯化法、低含量胰酶快速消化和差速贴壁法综合运用,所得许旺细胞的纯度很高,可达98%以上,而且细胞所受到的外界因素影响也较少。结论:通过体外培养方法得到高纯度且受到的外界因素影响小的许旺细胞,可采用组织块反复种植纯化法、低含量胰酶快速消化和差速贴壁法综合运用的方法。
AIM:The Schwann cells can secrete growth factor and plays an important role in the regeneration of peripheral nerve, and the premise of its study is to obtain high purified Schwann cells.The aim of this paper is to explore the effective method for obtaining the high purified Schwann cells from the newborn SD mice. METHODS:The repeated implantation,rapid trypsinization,and differential adhesi on were used. RESULTS:The Schwann cells obtained by using repeated implantation,rapid trypsi nization, and differential adhesion ways were high in purification,which could r each to more than 98%,and were little affected by non experimented factors. CONCLUSION:The high purified Schwann cells with little effect by non experim ental factors obtained by culture in vitro can be obtained by using repeated exp lantation,rapid trypsinization,and differential adhesion methods.
出处
《中国临床康复》
CAS
CSCD
北大核心
2005年第2期58-59,共2页
Chinese Journal of Clinical Rehabilitation
基金
广东省科学技术研究基金资助(B2001065)~~
