摘要
目的研究激活剂蛋白_1家族成员FosB,c_Fos,c_Jun,JunD,JunB,Fra_1和Fra_2对不同大小的流体剪切力的生理响应。方法对新生SD大鼠颅盖骨中分离出的成骨细胞施加流体剪切力,分成4组,每组加载的水平剪切力大小分别为0.8 Pa,1.2 Pa,1.4 Pa及1.6 Pa。每组在加载剪切力0 min,10 min,15 min,30 min,60 min后分别用逆转录聚合酶链反应测试FosB,c_Fos,c_Jun,JunD,JunB,Fra_1和Fra_2 mRNA的表达。结果FosB,c_Fos,c_Jun,JunD和JunB在水平剪切力加载15 min时表达明显增高(P<0.05),Fra_1和Fra_2在流体剪切力刺激后都有增高,但是各个时间组之间却没有统计学差异(P>0.05)。当剪切力为1.2 Pa时,FosB,c_Fos,c_Jun,JunD和JunB mRNA表达明显高于其他各组和空白对照组(P<0.05)。结论FosB,c_Fos,c_Jun,JunD,JunB,Fra_1和Fra_2等参与了力学刺激引发细胞响应的过程。当成骨细胞受到外界力学刺激后,激活剂蛋白_1在外界信号刺激引起的信息传递级联反应中起重要的偶联作用,充当核内第三信使和基因转录调控的分子开关。
Objective To observe activating protein- 1 （ AP- 1 ） members in response to changes of wall-shear stress in osteoblastic cells in vitro. Methods Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and subcultured. The third generation cells were subjected to wall-shear stress of 0.8 Pa, 1.2 Pa, 1.4 Pa and 1.6 Pa separately. Gene expression of the seven AP-1 members were studied before （0 h） and 10 min, 15 min,30 min,60 min after treated with wall-shear stress. Results The expression of FosB, c-Fos, c-Jun, JunD and JunB mRNA increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells compared to 0.8 Pa , 1.4 Pa and 1.6 Pa stress, and peaked at 15 min. Conclusion Mechanical environment changes in osteoblastic cells induced a dramatic induction of most of the AP-1 members.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2005年第5期380-384,共5页
West China Journal of Stomatology
基金
浙江省自然科学基金资助项目(491060_N20078)
关键词
流体剪切力
成骨细胞
激活剂蛋白-1
wall-shear stress
osteoblasfic cells
activating protein-1
