摘要
目的:观测流体剪切应力作用不同时间对鼠破骨细胞碳酸酐酶II(CAⅡ)mRNA表达的影响。方法:对破骨细胞爬片施加2.9dyne/cm2的流体剪切应力,按作用时间不同分为0min、15min、30min、60min和120min组,采用实时荧光定量PCR检测CAⅡmRNA表达水平,采用SPSS12.0软件包对数据进行单因素方差分析。结果:对照组、15min、30min、60min和120min组CAIImRNA表达量分别为7.88±0.09、11.14±0.12、15.83±0.18、1.94±0.02和1.37±0.01(E+5个拷贝数),任何两组间均见显著性差异(P<0.05)。结论:在试验条件下,随着加载时间的增加,CAⅡmRNA表达水平有增加趋势,至30min组达到峰值,随后表达水平有减小趋势,推测流体剪切应力作用下CAⅡmRNA表达水平存在一定时效关系,但具体作用机制有待进一步研究。
PURPOSE： To observe the effects of fluid shear stress on carbonic anhydrase II in rat osteoclasts. METHODS： Osteoclasts were subject to fluid shear stress for 0,15,30,60 and 120 minutes respectively, which was 2.9dyne/cm^2. mRNA level of carbonic anhydrase Ⅱ was detected through real-time fluorescent quantitation PCR. The data were analyzed with SPSS 12.0 software package. RESULTS： mRNA level of carbonic anhydrase II in control group, 15 minutes,30 minutes,60 minutes and 120 minutes group was 7.88±0.09, 11.14±0.12, 15.83±0.18, 1.94±0.02, and 1.37± 0.01 （E ＋5 copy number）, respectively. There was significant difference between any two groups （P〈0.05）. CONCLUSIONS： In this present study, mRNA level of carbonic anhydrase Ⅱ has an increasing tendency with various action times of fluid shear stress, it reaches to a peak value at 30 minutes. We speculated that there might be a certain time-effect relation between fluid shear stress and mRNA level of carbonic anhydrase Ⅱ, but this mechanism need be further studied. Supported by Educational Department Foundation of Zhejiang Province （Grant No.20080180） and Natural Science Foundation of Zhejiang Province（Grant No.Y2080340）.
出处
《上海口腔医学》
CAS
CSCD
2009年第3期302-306,共5页
Shanghai Journal of Stomatology
基金
浙江省教育厅基金(N20080180)
浙江省自然科学基金(Y2080340)~~
