摘要
目的探讨急性闭合性颅脑损伤(CCI)后胶质原纤维酸性蛋白(GFAP)变化分布,以及与核因子κB(NFκB)变化的关系。方法28只SD大鼠随机分为对照组(n=4)与损伤组(n=24),使用改良的液压冲击装置制作闭合性颅脑损伤模型,使用免疫组织化学分别测试损伤后不同时间点脑组织内NFκB与GFAP的变化。结果损伤后1h,散在的NFκB的免疫反应阳性细胞在病灶内可以观察到,损伤后24h,NFκB阳性反应遍布整个脑组织切片(吸光度90.2±2.2);损伤后6h,病灶内GFAP表达开始增强(吸光度29.08±1.69),损伤后5d,病灶内仍有大量的GFAP阳性表现(吸光度53.79±2.11),而NFκB的反应明显减少。结论NFκB部分参与了颅脑损伤后GFAP表达,抑制NFκB的表达,将一定程度上抑制GFAP表达。
Objective To investigate the changes in GFAP and the relationship with NF-κB after fluid percussion brain injury in rats. Methods Twenty-eight adult sprague -dawley rats were randomly divided into trauma group （n = 24） and control group （n = 4）. The fluid percussion brain injury model was made with modified strike equipment and the changes in GFAP and NF-κB at different time points after brain injury were detected by immunohistochemistry, Results The sporadic expression of NF-κB could be found in focus of disease after injury 1 h and sheeted all brain sections 24 h after injury （optical density 90.2±2.2）, decreased obviously 5 days after injury; The expression of GFAP was increased 6 h after injury （optical density 29.08 ± 1.69） and there was massive expression 5 days after injury 5 days, Conclusion GFAP might be involved in the process of brain injury and repair. There was a immanent relationship between GFAP and NF-KB, Inhibiting the expression of NF-κB can inhibit the expression of GFAP.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2005年第10期1257-1258,共2页
Chinese Journal of Experimental Surgery
基金
河北省教育厅博士基金攻关项目(B2004124)
