摘要
目的 明确急性创伤性脊髓损伤 (acutespinalcordinjury,ASCI)后胶质原酸性蛋白 (glialfibril laryacidicprotein ,GFAP)变化分布 ,以及与核因子κB(NF κB)变化的关系。方法 将 2 8只SD大鼠随机分为对照组与损伤组 ,制作脊髓损伤模型 ,使用免疫组化分别测试GFAP与NF κB在急性脊髓损伤后的变化。结果 ASCI后 6h ,白质内GFAP表达开始增强 ,ASCI后 5d ,灰白质内仍有大量的GFAP阳性表现 ;AS CI后 1h ,NF κB的免疫反应在病灶内和边缘部位可以探测到 ;ASCI后 5d ,NF κB的反应明显减少。结论 NF κB部分参与了脊髓损伤后GFAP表达 ,抑制NF κB的表达 ,将一定程度上抑制GFAP表达。
Objective To investigate the relationship between the expression of GFAP and NF κ B after spinal injury.Methods 28 rats were randomly divided into two groups,control group and injury group.The spinal injury model was made and the changes of GFAP and NF κ B after spinal injury were detected by immunohistochemistry.Results The expression of GFAP increased 6 hours after injury and still and massive expression 5 days after injury.The expression of NF κ B increased 1 hour after injury and decreased obviously 5 days after injyry.Conclusion GFAP joins the process of spinal cord injury and repair.GFAP and the activity of NF κ B has immanent relation.Inhibiting the activity of NF κ B can inhibit the expression of GFAP.
出处
《河北医药》
CAS
2002年第10期772-772,共1页
Hebei Medical Journal
