摘要
目的研制一种大鼠液压脑损伤实验装置。方法该装置由高压气体、储液、控制及检测等系统组成。测试其稳定性并术后1h给大鼠行神经功能评分，术后1、6、12、24h及3、7d用干湿重法测定损伤脑组织含水量、用光镜进行组织学观察。结果稳定性检测显示，相同设定压力下可获得等时程、峰值稳定的液压打击压力，且不同设定压力与获得的液压打击压力呈直线正相关（r=0．999，P〈0．05）。术后1h脑损伤各亚组之间神经功能评分差异无统计学意义（P〉0．05）。损伤脑组织含水量变化趋势及病理学变化过程与文献报道一致。结论该装置可精确定时、定量，而且制作的动物模型具有较好的稳定性及可重复性。
Objective To design an apparatus for fluid percussion brain injury in rats. Methods This apparatus was composed of high pressure gas systems, liquid storage systems, control and detection sys- tems and other components. The system stability was detected and neural functional scores of all animals were tested 1 h after operation. The water content and histological changes were observed at 1 h, 6 h, 12 h, 24 h, 3 day, 7 day after operation. Results Stability detection showed the coincident fluid percussion pressure of equal time-course and peak value was obtained with identical pressure set by distribution valve and the linear regression analysis indicated they had a positive correlation （r = 0. 999 ,P 〈 0. 05 ）. No significance was found in neural functional scores between the subsets of brain injury group 1 h after operation. The tendency of water content and histological changes was pro, Jed coincident with those reported by literatures. Conclusion Experimental results show that the apparatus can be accurately timed and quantified, and animal models be produced stabily and repeatedly.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第5期749-750,共2页
Chinese Journal of Experimental Surgery
基金
河北省自然科学基金资助项目（C2008001084）
河北省科技厅资助项目（08206123D）
关键词
液压脑损伤
装置
大鼠
Fluid percussion brain injury
Apparatus
Rat
