摘要
通过正交试验确定黑曲霉的最佳培养条件。由培养的黑曲霉湿菌体制备几丁质，最经济的方法是电解法；制备壳聚糖则采用间歇提取法，所得干燥壳聚糖产品的壳聚糖含量为９２．２９％，游离氨基为９６．１１％，１％壳聚精的１％醋酸溶液运动粘度为５．３３ｘ１０￣（－６）ｍ￣２／ｓ，粘均分子是为８．０２４ｘ１０￣４，灰分为９．２４％，产品得率为９．７２％，探讨了从柠檬酸厂废弃酸渣中分离黑曲霉菌丝体的方法，得率为３０％左右。
This paper first determined the best condition of culturing Aspergillus ni-ger,The economic way for obtaining chitin from the mycelium was electrolysis,the methodof preparing chitosan was to treat the mycelium two times with high concentrated NaOHsolution at 135℃. Purity of such chitosan product was 92.29%, free NH_2 was 96.01%,visco-sity was 5.33x10￣(-6) m￣2/s,viscosity-average molecular weight was 8.024x10￣4,ash was 9.24%。This paper also investigated to seperate mycelium of Aspergillus niger from the wastematerial of citric acid production plant. Yield of mycelium reached 30% r so.
出处
《微生物学通报》
CAS
CSCD
北大核心
1995年第4期200-203,共4页
Microbiology China
关键词
黑曲霉
几丁质
壳聚糖
Aspergillus niger,chitin, chitosan
