摘要
目的 苯并 [a]芘 (BaP)、铅及其联合作用对体外神经元的毒性及细胞凋亡率的影响。方法 取 8日龄SD大鼠小脑粒细胞培养 ,按以下分组进行处理 :空白对照组 ;溶剂对照组 ;低浓度铅染毒组 ;高浓度铅染毒组 ;低浓度BaP染毒组 ;高浓度BaP染毒组 ;低浓度铅 +低浓度BaP染毒组 ;低浓度铅 +高浓度BaP染毒组 ;高浓度铅 +低浓度BaP染毒组 ;高浓度铅 +高浓度BaP染毒组 ;高、低浓度的BaP染毒分别为BaP5 0 μmol/L +S9-mix和BaP5 μmol/L +S9-mix ,高、低浓度的铅染毒分别为 5 0和 5 μmol/L的PbAc ,溶剂对照组用等量DMSO平行处理 ,空白对照组不作处理。染毒 90min ,胰酶消化法收集标本。台盼蓝染色法检测存活率 ;DNA断点末端核糖核酸转移酶地高辛标记(TUNEL)法检测大鼠小脑粒细胞凋亡率 ,并对细胞存活率与凋亡率进行相关分析。结果 各染毒组细胞存活率均低于对照组 ,高剂量组降低程度高于低剂量组 ,联合染毒组降低程度高于单独染毒组 (P <0 0 5～P <10E - 7)。各染毒组细胞凋亡率显著升高 ,高剂量组升高程度高于低剂量组 ,联合染毒组升高程度高于单独染毒组 (P <0 0 0 1～P <10E - 9)。细胞凋亡率与存活率间呈负相关 (r =- 0 76 12 5 ,P <0 0 5 )。结论 BaP、铅均有一定的体外神经毒性 ,二者联合作用可使毒性?
Objective To study on the in vitro toxicity and cell apoptosis of benzo[a]pyrene or/and lead-exposed rat cerebellar granule neuro ns.Methods Primary cultures of rat cerebellar neurons were p repared from 8-day-old pups,there were 10 groups of cultured neurons in each ite m of experiments:standard control group;vehicle control group;low dose lead grou po;high dose lead group;low dose BaP group;high dose BaP group;low dose BaP+low dose lead group;low dose lead+high dose BaP group;high dose lead+low dose BaP gr oup;high dose lead+high dose BaP group.Low and high dose BaP group neurons were administrated with BaP dissolved in DSMO at 5 and 50?μmol/L,the vehicle contro l group was given DSMO parale and the standard control group was not given addit ional treatment;low and high dose lead group were administrated with PbAc at 5 a nd 50?μmol/L specially.Affter 90-minute administration,the neurons were collec ted by the method of trypsin digestion,the survival rates were detected by the m e thod of Tapian-blue dying.Detecting the apoptosis rates by the methods of TUNEL. The relationship between the survival rates and the apoptosis rates were calcula ted.Results The survival rates of all administrated groups we re significantly lower than that of control groups(P<0.05-P<10E-7).The ap optosis rates of all administrated groups were significantly higher than that of control groups (P<0.001-P<10E-9).There was an negative co-relationship b etween the survival rates and the apoptosis rates (r=-0.761?25,P<0.05).Conclusion Both BaP and lead can damage cultured neurons, combine administration of two toxicants can do more serious damage.Cell apoptosi s may be one of the mechanisms by which BaP resultsed in toxicity on the culture d neurons.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第10期1203-1204,共2页
Chinese Journal of Public Health
基金
重庆医科大学校内基金
