摘要
目的 研究苯并 [a]芘 (BaP)、高温及其联合作用对小鼠脑组织热应激蛋白 70 (HSP70 )与热应激蛋白 90 β (HSP90 β)表达的影响。方法 将 5 6只昆明种小鼠随机分为 7组 (每组 8只 ) ,即空白对照组、溶剂对照组、低剂量BaP单独染毒组、高剂量BaP单独染毒组、单独高温处理组、高温处理 +低剂量BaP染毒组、高温处理 +高剂量BaP染毒组。低、高剂量BaP染毒分别给予BaP 0 5mg/kg和 5mg/kg ,用植物油作溶剂 ,每周 4次腹腔注射 ,溶剂对照组用植物油作平行处理 ,空白对照组不做处理。高温处理为每周4次 ,每次 4 1 5～ 4 2 0℃处理 2h。实验 8周后取各组小鼠脑组织制作混合匀浆 ,Westernblot法检测HSP70和HSP 90 β水平。 结果 HSP70在高温单独作用、低剂量BaP单独作用、高温与低剂量BaP联合作用下表达明显升高。HSP90 β仅在高温与低剂量BaP联合作用及高温与高剂量BaP联合作用下表达升高。 结论 不同的热应激蛋白在不同条件下对高温。
Objective To study the effects of benzopyrene(BaP)or/and heat on levels of heat stress proteins 70(HSP70) and heat stress proteins 90β(HSP90 β)in mice brain.Methods 56 mice were randomly divided into seven groups,i e ,standard control group,vehicle control group,low dose BaP group,high dose BaP group,heat group,heat+low dose BaP group,and heat+high dose BaP group.After 8 experiment weeks,the mice were sacrificed;their brains were removed and made into homogenates.Detect levels of HSP70 and HSP90 β in homogenates by the method of western blot.Results For HSP70 level,in the low dose BaP group,heat group and heat+low dose BaP group,HSP70 level was much higher than that of control groups.For HSP90 β level,in the heat+low dose BaP group and heat+high dose BaP group, HSP90 β level was higher than that of control groups.Conclusions Different HSPs under different conditions play preventive and marking roles on damaged brain tissues induced by hazards such as heat and BaP.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2004年第5期283-285,共3页
Industrial Health and Occupational Diseases
基金
重庆医科大学校内基金
