摘要
目的 研究苯并 [a]芘、铅单独及其联合作用对体外神经元毒性及胞核DNA的损伤。方法 取 8日龄SD大鼠小脑粒细胞培养 ,按以下分组进行处理 :①空白对照组 ;②溶剂对照组 (等量DMSO +S9 mix平行处理 ) ;③低浓度铅染毒组 (PbAc5 μmol L) ;④高浓度铅染毒组 (PbAc5 0 μmol L) ;⑤低浓度BaP染毒组(BaP5 μmol L +S9 mix) ;⑥高浓度BaP染毒组 (BaP5 0 μmol L +S9 mix) ;⑦低浓度铅 +低浓度BaP联合染毒组 ;⑧低浓度铅 +高浓度BaP联合染毒组 ;⑨高浓度铅 +低浓度BaP联合染毒组 ;⑩高浓度铅 +高浓度BaP联合染毒组。染毒 90分钟 ,胰酶消化法收集标本 ,台盼蓝染色法检测存活率 ;单细胞凝胶电泳法检测胞核DNA的损伤。结果 ①两种浓度的BaP、铅单独或联合染毒均可降低细胞存活率 (P <0 0 5 ,P <0 0 1)。②高浓度BaP单独染毒组 (第 6组 )、两种毒物联合染毒且其中 1种或 2种为高浓度组 (第 8、9、10组 )与对照组相比胞核DNA损伤程度均有显著差异 (P <0 0 1)。结论 ①BaP、铅均有一定的体外神经毒性 ,二者联合作用可使毒性增强。②胞核DNA损伤可能是BaP引起体外神经毒性的机制之一。
Objective To study the effects on the survival rates and DNA damage of benzo[a]pyrene or lead alone or in combination in rat neurons in vivo Methods Primary cultures of rat cerebellar neurons were prepared from 8-day-old pups, 10 groups cultures in each experiments were used, ①control group, ②vehicle group(Equal volume of DMSO+S9-mix), ③low dose lead group(PbAc 5μmol/L),④high dose lead group(PbAc 50μmol/L),⑤low dose BaP group(BaP 5μmol/L+S9-mix), ⑥high dose BaP group(BaP 50μmol/L+S9-mix), ⑦low dose lead + low dose BaP group, ⑧low dose lead + high dose BaP group, ⑨high dose lead + low dose BaP group,⑩high dose lead + high dose BaP group. After 90 min administration, the neurons were collected by the method of trypsin digestion, the survival rates were detected by the method of Tapian-blue dying, Detect cracks on the DNA straps by the method of SCGE. Results ①The survival rates of all the administrated groups were significantly lower than that of control groups(P<0.05,P<0.01). ②In the high-dose BaP group(group 6), groups combine administrated with BaP and lead, one or two of them at high dose (groups 8,9,10), the DNA damage were more serious than that of control groups(P<0.01). Conclusion ①Both BaP and lead with some vivo neurotoxicity, the effects of two toxicants can do concord. ②Neuron DNA damage may be one of the mechanisms by which BaP result in toxicity on the cultured neurons.
出处
《卫生研究》
CAS
CSCD
北大核心
2004年第6期660-662,共3页
Journal of Hygiene Research
关键词
苯并[a]芘铅
体外神经元DNA损伤
benzo[a]pyrene, lead, cultured rat cerebellar granule neurons, DNA damage
