摘要
将地锦和五叶地锦杂交,未能获得杂交种。对人工辅助自交和杂交后,花粉萌发和花粉管生长情况进行了荧光显微观测。观察发现:人工辅助自交后,花粉粒可在各自柱头表面正常萌发,花粉管可伸入花柱内。将地锦和五叶地锦杂交时,多数花粉粒不能在柱头表面萌发,或者花粉管呈卷曲、先端膨大、破裂等异常现象。授粉后2～48h的花柱中,未观察到花粉管。由此推断,地锦和五叶地锦之间的不亲和发生在柱头表面。
The hybrids failed to get when crossing P.tricuspidata with P.quinquefolia.Germination of pollen and growth of pollen tube were studied by fluorescence microscope observation.The findings are as follows:pollens of P.tricuspidata and P.quinquefolia could germinate on the surface of their stigmas,respectively.Pollen tubes could grow into styles.While crossing P.tricuspidata with P.quinquefolia,most of the pollens could not germinate on the stigma.Even if germinated,the pollen tubes showed some abnormity such as twinning,tip inflation and bursting.2～48 h after pollinating,pollen tube was not observed in the styles of mother parents.It can be concluded that the cross-incompatibility was taken place on the surface of stigma.
出处
《林业科学研究》
CSCD
北大核心
2005年第1期52-56,共5页
Forest Research
基金
863项目"地锦(爬山虎)种质资源创新及优良品种培育"(2001AA244031)
关键词
地锦
五叶地锦
种间杂交
不亲和性
Parthenocissus tricuspidata:Parthenocissus quinquefolia
interspecific hybridization
cross-incompatibility
