摘要
从雌、雄两方面来研究科间杂种银榆杨(银白杨×(白榆+新疆杨失活花粉))的有性生殖能力。(1)以新疆杨、辽宁杨和辽胡3号杨为对照,采用花序数量、花粉量及花粉生命力3个指标评价银榆杨的雄性生殖能力,测定花粉生命力采用醋酸洋红染色、培养基培养、杂交试验3种方法。结果表明,在参试品种中,银榆杨雄花序数量最大,花粉量最小,花粉含水率最低。银榆杨花粉在培养基上萌发时花粉管出现扭曲、分叉等畸形现象,占萌发花粉的30%。以银榆杨为父本进行杂交育种可能出现杂交不亲和现象。(2)以银白杨为对照,用杂交法研究银榆杨雌性孕性。结果表明,银榆杨雌性可育,但与不同派品种的亲和力不同。最后,对银榆杨花粉管在培养基上萌发存在畸形的原因进行了讨论,对银榆杨应用于杂交育种提出建议。
An out-crossing experiment bettween Populus alba L. X （Ulmus pumila L. ＋DEAD P. alba L. var. pyramidalis） was conducted. By using techniques of pollen inducement and puerile ovary culture, the out-crossing barrier was conquered, and hybrid seedling of P. alba X U. pumila were successfully produced. The hybrids of P. alba X（U. purnila L. ＋DEAD P. alba L. var. pyramidalis） were named P. X alba L. ‘yinyu’. Sterility usual occurred in hybridization of distant taxa. Sexual reproduction of interfamilial hybrids is concerned widely. Sexual reproduction of P. X alba L. ‘yinyu’ was studied in male and female from the following aspects： （1） The number of inflorescences, weight of pollen and vitality of pollen were recorded to evaluate sexual reproduction. P. X Liaonignica sp. Nov. , P. alba L. var. pyrarnidalis and （P. sirnonii X P. euphratica） X P. nigra L. var. italica（Moench. ） were used as controls. Pollen viability was detected by aceto carmine, culture medium and mating. The results indicated that the number of inflorescences of P. X alba L. ‘yinyu’ was the greatest, while the weight and vitality of pollen were the lowest among all tested samples. There were about 30 % pollen tubes that were tortuose and forficate in culture medium. Colligating the results of dye and crossing, cross incompatibility was possible when P. X alba L. ‘yinyu’ was the male parent; （2） an pollination test of P. X alba L. ‘yinyu’ was conducted and P. X alba L. was used as a control. Female P. X alba L. ‘yinyu’ was pollinated, but showing difference affinities with different male parents. At last, the causes for pollen tube abnormality of P. X alba L. ‘yinyu’ were discussed. Some recommendations were also made on using P. × alba L. ‘yinyu’as a parent.
出处
《西北林学院学报》
CSCD
北大核心
2010年第2期56-60,共5页
Journal of Northwest Forestry University
基金
辽宁省自然科学基金项目(20062211)
关键词
有性生殖
花粉管畸形
银白杨
白榆
sexual reproduction
pollen tube abnormality
Populus alba L.
Ulmus pumila L.
