摘要
本试验以秋水仙素为诱导剂,对石斛杂交种Dendrobium utopia‘Messenger’×Den.whiterabbit‘Sakurahime’的二倍体试管苗进行了多倍体诱导、细胞学观察及染色体鉴定等研究。结果表明,当秋水仙素浓度为0.4-0.6mg/L时石斛的诱导率较高,最高时可达62.2%。变异植株的叶片变短变厚,假鳞茎明显增粗。在显微镜下观察到变异株的气孔长度和宽度约增加21.44%和28.30%,保卫细胞的长度和宽度约增加30.88%和24.98%。
The tube seedlings of dendrobium hybrids was induced by colchicines, and morphology observation and cytology identification of the polyploid seedlings were carried out. The results indicated that the mutation rate could reach 62.2 % under 0.04 % - 0.06 % colchieines treatment. The leaves of mutants were shorter and thicker than normal plants and the pseudobulb enlarged obviously. Microscope observation indicated that the length and width of stoma increased 21.44 % and 28.30 %, and the length and width of guard cells increased 30.88 % and 24.98 %, respectively.
出处
《核农学报》
CAS
CSCD
北大核心
2009年第3期413-417,共5页
Journal of Nuclear Agricultural Sciences
基金
国家林业局948项目(2005-4-37,2006-4-C07)
中央级公益性科研院所基本科研业务费专项资金项目(RISF6801)
关键词
石斛
组织培养
秋水仙素
多倍体
Dendrobium
tissue culture
colchicines
polyploid
