摘要
从105株霉菌和酵母菌中筛选到一株木聚糖酶活力较高的棒曲霉(Aspergillua clavatus)菌株22。该菌株适宜的产酶培养基为(g/1):蔗渣半纤维素30,NH_4NO_3 5,酵母膏5,麸皮10,吐温801和少量无机盐,初始pH5.5。最适的孢子接种量为4.9×10~6个/ml。在上述培养基中28℃振荡培养72h.木聚糖酶活力可达335.9u/ml。酶反应的最适温度为50℃;最适pH为4.8,在pH 6-11酶活性稳定。50℃保温1h,酶活力剩余72.6%;在8℃下放置9天,剩余90%。多种糖以及Na^+、Ca^(2+)、Zn^(2+)等离子可提高酶的水解活性,而Co^(2+)、Cu^(2+)等离子则抑制酶的活性。
A high xylanase producing strain 22 of Aspergillus clavatus was screened from 105 strains of molds and yeasts. The suitable medium consisted of (g/L): bagasse hemicellulose 30, NH_4NO_3 5, yeast extract 5, wheat bran 10, Tween 80 1 and a small quantity of other minerals; initial pH 5.5. Theoptimalsporeinoculumwas4.9X10^6spores/ml (final concentration). Theactivity of xylanase was as high as 335.9 U/ml in shake-flask experiment at 28℃ for 72 h. The optimal temperature and pH for xylanase reaction were 50℃ and pH 4.8. 72.6% of its original activity was remained after incubation at 50℃ for 1 h, and 90% of the enzym activity was observed upon storage at 8℃ for 9 days . Sugars. Na^+. Ca^(2+). and Zn^(2+) increased its activity wherease Co^(2+)and Cu^(2+) inhibited it.
出处
《微生物学通报》
CAS
CSCD
北大核心
1993年第6期331-335,共5页
Microbiology China
关键词
棒曲霉
木聚糖酶
真菌
Aspergillus clavatus
Xylanase
