摘要
利用亨氏厌氧技术从沤麻塘底泥等厌氧生境中分离筛选到Ｃｌｏｓｔｒｉｄｉｕｍ属的Ｎ２、Ｙ４、Ｚ２－８３株厌氧细菌。研究结果表明，其产酶最适ｐＨ为７．０～７．４，最适温度为３０℃，最佳碳源为淀粉，最佳氮源为豆饼粉。Ｎ２、Ｙ４、Ｚ２－８的果胶酶活分别为９１３ｕ、９７３ｕ、９２７ｕ，半纤维素酶活分别为１３７ｕ、１４０ｕ、１７０ｕ，纤维素酶活分别为０ｕ、３ｕ、０ｕ，产酶高峰时间在７２ｈ左右。３个菌株混合发酵红麻皮、构皮等韧皮纤维，５ｄ可成浆。打浆后做手抄片，红麻皮浆手抄片白度为４０．５，裂断长为３５２９ｍ；构皮浆手抄片白度为２２．７，裂断长为２９９８ｍ。
Clostridium isolates were obtained from the anaerobic environment of retting kenaf pool by Hungate anaerobic culturing technique and designated as strains N 2, Y 4, Z 2-8 . The basic cultural conditions for the above strains were tested. It was found that the optimal pH was 7.0～7.4, the optimal carbon source being amylum, the optimal nitrogen source being beancake powder. The retting was accomplished within 5 days. The breaking length of the handsheets made from the hozo white bast fibre was 2998 m, it′s brightness being 22.7. The pectinase activity of N 2, Y 4, Z 2-8 was 931 u, 973 u, 927 u; the hemicellulase activity was 137 u, 140 u, 170 u respectively; the cellulase activity was 0 u, 3 u, 0 u respectively.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1998年第3期241-245,共5页
Journal of Huazhong Agricultural University
