摘要
1目的 研究维生素 C对尿酸测定的干扰及机制。 2方法 采用尿酸酶 -过氧化物酶 (Uricase-PAP)法测定尿酸。 3结果 对尿酸浓度相同、维生素 C浓度不同的系列标本进行测定 ,所测尿酸浓度随着维生素 C浓度的升高而降低。测定不含尿酸的过氧化氢溶液时 ,所测尿酸浓度随着过氧化氢稀释度的增大而降低 ;当过氧化氢浓度相同时 ,所测尿酸浓度随着维生素 C浓度的增高而降低。4结论 维生素
Objective To study the interference and mechanism of vitamin C on determination of uric acid. Methods Uric acid concentrations were determined with Uricase PAP method. Results When a series of samples containing the same concentration of uric acid but different concentrations of vitamin C were determined,the uric acid concentrations decreased with the increase of vitamin C concentrations.When uric acid free hydrogen peroxide solutions were determined,the uric acid concentrations decreased with dilutions of hydrogen peroxide;when hydrogen peroxide concentration is constant,the uric acid concentrations decreased with increase of vitamin C concentrations in the solutions.Conclusion Vitamin C interferes with the determination of uric acid by reduction of both the intermittent product hydrogen peroxide and the final product quinoneimine. [
出处
《华北煤炭医学院学报》
2001年第1期3-4,共2页
Journal of North China Coal Medical College
关键词
尿酸
测定
维生素C
干扰
Uric acid
Determination
Vitamin C
Interference
